PE Mouse Anti-Human Granzyme B

Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info

BD Pharmingen™ PE Mouse Anti-Human Granzyme B

Name: PE Mouse Anti-Human Granzyme B
Brand: BD Pharmingen™
SKU: 561142

克隆 GB11

(RUO)

Flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with Alexa Fluor® 647 Mouse Anti-Human CD8 (Cat. No. 557708) and either PE Mouse Anti-Human Granzyme B Antibody (Cat. No. 561142, Left Panel) or PE Mouse IgG1 Isotype Control (Cat. No. 555749, Right Panel) by using BD Biosciences Pharmingen's Staining Protocol. Two-color flow cytometric dot plots showing the correlated expression patterns of CD8 and Granzyme B or Mouse IgG1 Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.

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科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: GZMB; Granzyme-2; CCPI; CGL1; CSPB; CTLA1; CTSGL1; GRB; HLP; SECT

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Granzyme B

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_10561690

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

配套商品

Stain Buffer (FBS) RUO

尺寸 500 mL 品类编号 554656

Fixation Buffer RUO

尺寸 100 mL 品类编号 554655

Perm/Wash Buffer RUO

尺寸 100 mL 品类编号 554723

PE Mouse IgG1, κ Isotype Control RUO

尺寸 100 Tests 品类编号 555749

Alexa Fluor® 647 Mouse Anti-Human CD8 RUO

尺寸 100 Tests 品类编号 557708

561142 Rev. 1

抗体详情

GB11

The GB11 antibody specifically reacts with human granzyme B, a serine protease of approximately 32 kDa. Granzyme B is stored in the granules of cytotoxic T lymphocytes and NK cells along with the pore-forming protein perforin. In the classic model of target cell lysis, perforins create holes in the target cell membrane allowing entrance of granzymes. Granzyme B has been shown to act on specific substrates including caspase-3, -7, -9, and -10 which in turn give rise to enzymes that mediate apoptosis. Granzyme B may also be involved in the hydrolysis of extracellular matrix components. Detectable levels of granzyme B have been detected in sera from healthy volunteers. The immunogen used to generate the GB11 hybridoma was human granzyme B isolated from an NK cell line.

561142 Rev. 1

格式详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PE

激发源: Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源: 496 nm, 566 nm

最多发射源: 576 nm

561142 Rev.1

报价单和参考

研发参考 (8)

Hamann D, Baars PA, Rep MH. Phenotypic and functional separation of memory and effector human CD8+ T cells. J Exp Med. 1997; 186(9):1407-1418. (Clone-specific: Flow cytometry). 查看参考
Ronday HK, van der Laan WH, Tak PP et al. Human granzyme B mediates cartilage proteoglycan degradation and is expressed at the invasive front of the synovium in rheumatoid arthritis. Rheumatology (Oxford). 2001; 40:55-61. (Biology). 查看参考
Smyth MJ, Kelly JM, Sutton VR et al. Unlocking the secrets of cytotoxic granule proteins. J Leukoc Biol. 2001; 70:18-29. (Biology). 查看参考
Spaeny-Dekking EH, Hanna WL, Wolbink AM et al. Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998; 160:3610. (Biology). 查看参考
Trapani JA, Klein JL, White PC, and Dupont B. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc Natl Acad Sci U S A. 1988; 5:6924-6928. (Biology). 查看参考
Trapani JA, Smyth MJ, Apostolidis VA, Dawson M, and Browne KA. Granule serine proteases are normal nuclear constituents of natural killer cells. J Biol Chem. 1994; 269:18359-18365. (Biology). 查看参考
Wever PC, Van Der Vliet HJ, Spaeny LH . The CD8+ granzyme B+ T-cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells. Immunology. 1998; 93(3):383-389. (Clone-specific: Flow cytometry). 查看参考
ten Berge IJ, Wever PC, Rentenaar RJ. Selective expansion of a peripheral blood CD8+ memory T cell subset expressing both granzyme B and L-selectin during primary viral infection in renal allograft recipients. Transplant Proc. 1998; 30(8):3975-3977. (Biology). 查看参考

查看所有文件 (8)

561142 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.