PE Mouse Anti-Human CD307a (FCRL1)

BD Pharmingen™ PE Mouse Anti-Human CD307a (FCRL1)

Name: PE Mouse Anti-Human CD307a (FCRL1)
Brand: PE Mouse Anti-Human CD307a (FCRL1)
SKU: 566457

克隆 E3 (RUO)

Two-color flow cytometric analysis of CD307a (FCRL1) expression on human peripheral blood lymphocytes. Human peripheral blood was stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human CD307a (FCRL1) antibody (Cat. No. 566457; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color contour plots showing the correlated expression of CD19 versus CD307a (FCRL1) [Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System..

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实验方案

科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: FCRL1; FCRH1; IFGP1; IRTA5; Immunoglobulin superfamily Fc receptor gp42

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human FCRL1 cDNA

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Workshop Number: IX 20

RRID: AB_2739736

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Stain Buffer (FBS) RUO

尺寸 500 mL 品类编号 554656

Stain Buffer (BSA) RUO

尺寸 500 mL 品类编号 554657

Lysing Buffer RUO

尺寸 100 mL 品类编号 555899

Lysing Solution 10X Concentrate CFDA_IVD

尺寸 100 mL 品类编号 349202

PE Mouse IgG1, κ Isotype Control RUO

尺寸 0.1 mg 品类编号 554680

APC Mouse Anti-Human CD19 RUO

尺寸 100 Tests 品类编号 555415

查看详情

566457 Rev. 1

抗体详情

The E3 monoclonal antibody specifically recognizes CD307a which is also known as Fc receptor-like protein 1 (FCRL1), Fc receptor homolog 1 (FCRH1), or Immune receptor translocation-associated protein 5 (IRTA5). CD307a (FCRL1) is a type I transmembrane glycoprotein that belongs to the IRTA gene family within the Ig gene superfamily. It has three extracellular Ig-like domains, a glutamic acid residue in its transmembrane region, and two consensus immunoreceptor tyrosine-based activation motifs (ITAMs) in its cytoplasmic domain. CD307a (FCRL1) bears sequence homology to classical Fc receptors. CD307a (FCRL1) is first expressed on pre-B cells and increases with maturation being expressed on naïve and memory B cells. It is downregulated by activated germinal center (GC) B cells. It may also be expressed by B cell-derived leukemia and lymphoma cells. CD307a may play key roles in B cell activation, differentiation and effector functions.

566457 Rev. 1

格式详情

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: PE

激发源: Yellow-Green 488 nm, 532 nm, 561 nm

最多激发源: 496 nm, 566 nm

最多发射源: 576 nm

566457 Rev.1

报价单和参考

View product citations for antibody "566457" on CiteAb

研发参考 (3)

Du X, Nagata S, Ise T, Stetler-Stevenson M, Pastan I. FCRL1 on chronic lymphocytic leukemia, hairy cell leukemia, and B-cell non-Hodgkin lymphoma as a target of immunotoxins.. Blood. 2008; 111(1):338-43. (Immunogen: Cytotoxicity, Flow cytometry, Functional assay). 查看参考
Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol Lett. 2011; 134(2):113-121. (Biology). 查看参考
Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P. New B-cell CD molecules. Immunol Lett. 2011; 134(2):104-112. (Biology). 查看参考

566457 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.