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BD Pharmingen™ PE Mouse Anti-Human CD194
克隆 1G1 (RUO)
Flow cytometric detection of CD194 (CCR4) on human peripheral lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stained with the PE Mouse Anti-Human CD194 antibody in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. No. 555346 , left panel) or with a FITC Mouse Anti-Human CD8 antibody (Cat. No. 555366, right panel). The cells were gated on lymphocytes and quadrant markers for the bivariate dot plots were set based on staining with a PE Mouse IgG1 κ Isotype Control (Cat. No. 555749).
Flow cytometric detection of CD194 (CCR4) on human peripheral lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stained with the PE Mouse Anti-Human CD194 antibody in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. No. 555346 , left panel) or with a FITC Mouse Anti-Human CD8 antibody (Cat. No. 555366, right panel). The cells were gated on lymphocytes and quadrant markers for the bivariate dot plots were set based on staining with a PE Mouse IgG1 κ Isotype Control (Cat. No. 555749).
Flow cytometric detection of CD194 (CCR4) on human peripheral lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stained with the PE Mouse Anti-Human CD194 antibody in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. No. 555346 , left panel) or with a FITC Mouse Anti-Human CD8 antibody (Cat. No. 555366, right panel). The cells were gated on lymphocytes and quadrant markers for the bivariate dot plots were set based on staining with a PE Mouse IgG1 κ Isotype Control (Cat. No. 555749).
Flow cytometric detection of CD194 (CCR4) on human peripheral lymphocytes. Human peripheral blood mononuclear cells (PBMC) were stained with the PE Mouse Anti-Human CD194 antibody in conjunction with either a FITC Mouse Anti-Human CD4 antibody (Cat. No. 555346 , left panel) or with a FITC Mouse Anti-Human CD8 antibody (Cat. No. 555366, right panel). The cells were gated on lymphocytes and quadrant markers for the bivariate dot plots were set based on staining with a PE Mouse IgG1 κ Isotype Control (Cat. No. 555749).
商品详情
BD Pharmingen™
CCR4; C-C chemokine receptor type 4; CMKBR4; K5-5
Human (QC Testing)
Mouse C57BL/6 IgG1, κ
Human CCR4 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
HCDM 2006
AB_394054
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
准备和存储
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561110 Rev. 2
抗体详情
1G1
The 1G1 monoclonal antibody specifically binds to CD194, also known as the human CC Chemokine Receptor type 4 (CCR4). CCR4 is expressed on activated Th2 cells, regulatory T cells, activated NK cells, basophils, monocytes and platelets. CCR4 is a seven-transmembrane, G-protein-coupled receptor, and is the specific receptor for CC chemokines, CCL22/MDC/Macrophage-Derived Chemokine and CCL17/TARC/Thymus and Activation-Regulated Chemokine. It has been reported that CCR4 mRNA is expressed mainly in the thymus and spleen. The human CCR4 gene has been mapped to chromosome 3p24. The purified form of this antibody has been reported not to be a neutralizing antibody. The immunogen used to generate the 1G1 hybridoma has been reported to be human CCR4 transfected L1.2 mouse lymphoma cells.
561110 Rev. 2
格式详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
561110 Rev.2
报价单和参考
View product citations for antibody "561110" on CiteAb
研发参考 (9)
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Andrew DP, Ruffing N, Kim CH, et al. C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential. J Immunol. 2000; 166(1):103-111. (Biology). 查看参考
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Bonecchi R, Bianchi G, Bordignon PP, et al. Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s. J Exp Med. 1998; 187(1):129-134. (Biology). 查看参考
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Campbell JJ, Haraldsen G, Pan J, et al. The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature. 1999; 400(6746):776-780. (Biology). 查看参考
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D'Ambrosio D, Iellem A, Bonecchi R, et al. Selective up-regulation of chemokine receptors CCR4 and CCR8 upon activation of polarized human type 2 Th cells.. J Immunol. 1998; 161(10):5111-5. (Biology). 查看参考
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Imai T, Baba M, Nishimura M, Kakizaki M, Takagi S, Yoshie O. The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4. J Biol Chem. 1997; 272(23):15036-15042. (Biology). 查看参考
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Imai T, Chantry D, Raport CJ, et al. Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J Biol Chem. 1998; 273(3):1764-1768. (Biology). 查看参考
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Power CA, Meyer A, Nemeth K, et al. Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line. J Biol Chem. 1995; 270(33):19495-19500. (Biology). 查看参考
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Sallusto F, Lenig D, Mackay CR, Lanzavecchia A. Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes. J Exp Med. 1998; 187(6):875-883. (Biology). 查看参考
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Samson M, Soularue P, Vassart G, Parmentier M. The genes encoding the human CC-chemokine receptors CC-CKR1 to CC-CKR5 (CMKBR1-CMKBR5) are clustered in the p21.3-p24 region of chromosome 3. Genomics. 1996; 36(3):522-526. (Biology). 查看参考
561110 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.