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Reagents
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蛋白质印迹试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
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- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Analysis of eIF4E (pS209) in monocytes. Human peripheral blood mononuclear cells (PBMC) were either stimulated with 40 nM Phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich Cat. No. P8139) for 15 minutes (shaded histogram) or unstimulated (open histogram). The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes, then permeabilized (BD Phosflow™ Perm Buffer II, Cat. No. 558052) on ice for at least 30 minutes, and then stained with PE Mouse anti-elF4E (pS209, Cat. No. 560229). Monocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSArray™ bioanalyzer system. The specificity of mAb J77-925 was confirmed by western blot analysis using unconjugated antibody on lysates from control (lane 1) and PMA-treated (lane 2) PBMC. elF4E (pS209) is identified as a band of 25 kDa, with increased intensity in the treated lane.
BD™ Phosflow PE Mouse anti-elF4E (pS209)
BD™ Phosflow PE Mouse anti-elF4E (pS209)
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Either BD Cytofix™ fixation buffer or BD Phosflow™ Fix Buffer I may be used for cell fixation. Any of the three BD Phosflow™ permeabilization buffers may be used.
商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The eukaryotic translation Initiation Factor 4E (eIF4E) is a 25-kDa phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mRNA. eIF4E is the rate-limiting component for the initiation of cap-dependent translation by the eIF4F translation initiation complex, which is composed of eIF4E, eIF4G, and eIF4A. This complex promotes the unwinding of secondary structure at the 5' untranslated region of mRNA that is necessary to expose and locate the AUG initiation codon. Other functions of eIF4E have been identified, such as promoting the export of mRNAs that are involved in cell cycle progression from the nucleus and differentially regulating the translation of certain mRNAs in the cytoplasm. Three mechanisms for eIF4E regulation have been identified: Mnk1-mediated phosphorylation on serine 209 (S209) is required for eIF4E binding to the cap structure; over-expression of phosphorylated eIF4E can lead to increased cell proliferation, suppression of apoptosis, and a transformed phenotype; and interactions with nonphosphorylated eIF4E-binding proteins inhibit the formation of the eIF4F complex.
The J77-925 monoclonal antibody recognizes the phosphorylated S209 (pS209) of eIF4E.
研发参考 (5)
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Culjkovic B, Topisirovic I, Borden KLB. Controlling gene expression through RNA regulons. The role of the eukaryotic translation initiation factor eIF4E. Cell Cycle. 2007; 6(1):65-69. (Biology).
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Grolleau A, Kaplan MJ, Hanash SM, Beretta L, Richardson B. Impaired translational response and increased protein kinase PKR expression in T cells from lupus patients. J Clin Invest. 2000; 106(12):1561-1568. (Biology).
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Mendez R, Myers Jr MG, White MF, Rhoads RE. Stimulation of protein synthesis, eukaryotic translation initiation factor 4E phosphorylation, and PHAS-I phosphorylation by insulin requires insulin receptor substrate 1 and phosphatidylinositol 3-kinase. Mol Cell Biol. 1996; 16(6):2857-2864. (Biology). 查看参考
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Waskiewicz AJ, Flynn A, Proud CG, Cooper JA. Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. EMBO J. 1997; 16(8):1909-1920. (Biology). 查看参考
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Wendel H-G, Silva RLA, Malina A, et al. Dissecting eIF4E action in tumorigenesis. Genes Dev. 2007; 21:3232-3237. (Biology).
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