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- BD Rhapsody™ ATAC-Seq Assays
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Two-color analysis of CD55 expression on splenic B lymphocytes. C57BL/6 splenocytes were pre-incubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 (Cat. No. 553141/553142), stained with FITC anti-mouse CD45R/B220 (Cat. No. 553087/553088) and either PE Hamster IgG3, λ isotype control (Cat. No. 553980, left panel) or PE Hamster anti-Mouse CD55 (Cat. No. 58037, right panel). Dead cells were excluded by staining with propidium iodide (Cat. No. 556463), and the total viable leukocytes are displayed. The data demonstrates that CD55 is expressed on the majority of splenocytes, with the greatest density on B lymphocytes. Flow cytometry was performed on a BD FACScalibur™ flow cytometry system.
BD Pharmingen™ PE Hamster anti-Mouse CD55
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The RIKO-5 monoclonal antibody specifically recognizes the membrane-associated complement regulator CD55, also known as decay-accelerating factor (DAF). CD55 is expressed on the surface of erythrocytes and most splenic leukocytes, including B and T lymphocytes and CD11b-positive splenocytes, but it is not detected on thymocytes. Immunohistochemical staining (using a different monoclonal antibody) demonstrates that CD55 is expressed on vascular endothelium and many other cells types in a wide variety of organs. In the mouse, there are two isoforms of CD55, transmembrane and GPI-anchored, that are recognized by the RIKO-5 antibody. Both isoforms are able to protect cells from complement attack by inhibiting the deposition of C3 on antibody-sensitized cells, but the RIKO-5 antibody is not able to neutralize this activity. CD97, a member of the EGF-TM7 family that is widely expressed in mouse tissues, is another ligand for CD55.
研发参考 (7)
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Ahmad SR, Lidington EA, Ohta R, et al. Decay-accelerating factor induction by tumour necrosis factor-alpha, through a phosphatidylinositol-3 kinase and protein kinase C-dependent pathway, protects murine vascular endothelial cells against complement deposition. Immunology. 2003, October; 110(2):258-268. (Biology). 查看参考
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Leemans JC, te Velde AA, Florquin S, et al. The epidermal growth factor-seven transmembrane (EGF-TM7) receptor CD97 is required for neutrophil migration and host defense. J Immunol. 2004, January; 172(2):1125-1131. (Biology). 查看参考
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Lin F, Fukuoka Y, Spicer A, et al. Tissue distribution of products of the mouse decay-accelerating factor (DAF) genes. Exploitation of a Daf1 knock-out mouse and site-specific monoclonal antibodies. Immunology. 2001, October; 104(2):215-225. (Biology). 查看参考
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Miwa T, Maldonado MA, Zhou L, et al. (CD55) exacerbates autoimmune disease development in MRL/lpr mice. Am J Pathol. 2002, September ; 161(3):1077-1086. (Biology). 查看参考
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Miwa, T., X. Sun, et al. Characterization of glycosylphosphatidylinositol-anchored decay accelerating factor (GPI-DAF) and transmembrane DAF gene expression in wild-type and GPI-DAF gene knockout mice using polyclonal and monoclonal antibodies with dual or single specificity.. Immunology. 2001; 104:207-214. (Biology). 查看参考
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Ohta R, Imai M, Fukuoka Y, Miwa T, Okada N, Okada H. Characterization of mouse DAF on transfectant cells using monoclonal antibodies which recognize different epitopes. Microbiol Immunol. 1999; 43(11):1045-1056. (Biology). 查看参考
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Yamada K, Miwa T, Liu J, Nangaku M, Song WC. Critical protection from renal ischemia reperfusion injury by CD55 and CD59. J Immunol. 2004, March; 172(6):3869-3875. (Biology: Flow cytometry). 查看参考
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