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      FITC Rat Anti-Mouse T- and B-Cell Activation Antigen
      FITC Rat Anti-Mouse T- and B-Cell Activation Antigen
      Flow cytometric analysis for T- and B- Cell Activation Antigen in activated mouse spleen cells. Concanavalin A-stimulated (3 days) mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either FITC Rat IgM, κ Isotype Control (Cat No. 553942; dashed line histogram) or with the FITC Rat Anti-Mouse T- and B- Cell Activation Antigen antibody (Cat No. 553666/562080; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      FITC Rat Anti-Mouse T- and B-Cell Activation Antigen
      Flow cytometric analysis for T- and B- Cell Activation Antigen in activated mouse spleen cells. Concanavalin A-stimulated (3 days) mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with either FITC Rat IgM, κ Isotype Control (Cat No. 553942; dashed line histogram) or with the FITC Rat Anti-Mouse T- and B- Cell Activation Antigen antibody (Cat No. 553666/562080; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      商品详情
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      BD Pharmingen™
      GL7; GL-7; GL7 Antigen
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgM, κ
      In vitro-activated T-cell-depleted CBA/Ca mouse splenocytes
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394981
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
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      562080 Rev. 2
      抗体详情
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      GL7

      The GL7 antibody specifically recognizes the T- and B- Cell Activation Antigen which is also known as, the GL7 antigen. The GL7 antigen is a 35-kDa cell-surface protein that is expressed on T and B lymphocytes activated in vitro, on bone marrow Pre-B-II cells, germinal-center B cells, and the subpopulation of thymocytes that coexpress high CD3e levels.  The GL7 antibody recognizes an epitope containing nonsulfated α2-6-sialyl-LacNAc. There is strain variability with respect to GL7 antigen distribution on thymocytes and Con A-activated spleen cells. GL7 antigen expression is found to be higher on BALB/c mouse leucocytes than on C57BL/6 mouse counterparts. The GL7 antibody reportedly may crossreact with epitopes on molecules expressed by certain rat and human leucocyte subsets.

      562080 Rev. 2
      格式详情
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      FITC
      Blue 488 nm
      494 nm
      518 nm
      562080 Rev.2
      报价单和参考
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      研发参考 (5)

      1. Han S, Dillon SR, Zheng B, Shimoda M, Schlissel MS, Kelsoe G. V(D)J recombinase activity in a subset of germinal center B lymphocytes. Science. 1997; 278(5336):301-305. (Biology). 查看参考
      2. Han S, Zheng B, Schatz DG, Spanopoulou E, Kelsoe G. Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cells. Science. 1996; 274(5295):2094-2097. (Biology). 查看参考
      3. Han S, Zheng B, Takahashi Y, Kelsoe G. Distinctive characteristics of germinal center B cells. Semin Immunol. 1997; 9(4):255-260. (Biology). 查看参考
      4. Hathcock KS, Pucillo CE, Laszlo G, Lai L, Hodes RJ. Analysis of thymic subpopulations expressing the activation antigen GL7. Expression, genetics, and function. J Immunol. 1995; 155(10):4575-4581. (Biology). 查看参考
      5. Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Immunogen). 查看参考
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      562080 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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