The 7D2D3 monoclonal antibody specifically recognizes mouse Dendritic Cell Activating Receptor 2 (DCAR2), a C-type lectin encoded by the Clec4b1 gene. DCAR2 (Clec4b1) is a member of the dendritic cell immunoreceptor (DCIR) family, which contains four dendritic cell inhibitory receptors (DCIR1-4) and two activating receptors (DCAR1, 2). The extracellular region of DCAR2 (Clec4b1) has one C-type lectin carbohydrate recognition domain (CRD), that is highly similar to that of DCIR1, followed by a transmembrane region. Instead of a cytoplasmic Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) that can transmit inhibitory signals, DCAR2 associates with the Immunoreceptor Tyrosine-based Activating Motif (ITAM)-containing adaptor molecule, Fc Receptor γ (FcRγ). DCAR2 (Clec4b1) is expressed on restricted subsets of conventional dendritic cells in bone marrow and skin-draining lymph nodes. C-type lectins bind to carbohydrates in a calcium-dependent manner, and this receptor may be involved in the regulation of immune responses to bacterial infections.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.