Skip to main content Skip to navigation
BV421 Rat Anti-Mouse IL-17 Receptor B
BV421 Rat Anti-Mouse IL-17 Receptor B
Flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse IL-17 Receptor B antibody (Cat. No. 743815; solid line histogram) on mouse IL17rb-transfected 293F cells, with 293F untransfected control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Flow cytometric analysis using BD OptiBuild™ BV421 Rat Anti-Mouse IL-17 Receptor B antibody (Cat. No. 743815; solid line histogram) on mouse IL17rb-transfected 293F cells, with 293F untransfected control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
商品详情
Down Arrow Up Arrow


BD OptiBuild™
IL-17RB; IL-17B receptor; Il17rb; Evi27; Il17rb; IL-17ER; IL17RH1; IL-17Rh1
Mouse (Tested in Development)
Rat IgG1, κ
Mouse IL-17 Receptor B Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
50905
AB_2741776
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

推荐的实验流程

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

商品通知

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
743815 Rev. 2
抗体详情
Down Arrow Up Arrow
6B7

The 6B7 monoclonal antibody specifically binds to IL-17 Receptor B (IL-17RB), which is a single-pass type I transmembrane protein and a component of the heterodimeric receptor for IL-25 (also known as IL-17E). Several components of the IL-17RB signaling mechanism have been identified, including ACT1, TRAF4, SMURF2, DAZAP2, and STAT5. The cytoplasmic tails of both of the IL-25 Receptor components, IL-17RA and IL-17RB, and ACT1 contain the SEFIR (similar expression to fibroblast growth factor genes and IL-17R) domain that is essential for IL-25R-mediated signaling. Signaling through IL-17RB mediates Th2 immune responses and is responsible for pathogenesis in a murine model for asthma. IL-17RB is found on subsets of iNKT cells, Th2 cells, some myeloid cells in solid organs, and type 2 innate lymphoid cells (ILC2) in lung, peritoneum, mesenteric lymph nodes and bone marrow.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

743815 Rev. 2
格式详情
Down Arrow Up Arrow
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV421
Violet 405 nm
407 nm
423 nm
743815 Rev.2
报价单和参考
Down Arrow Up Arrow
View product citations for antibody "743815" on CiteAb

研发参考 (5)

  1. Hoyler T, Klose CS, Souabni A, et al. The transcription factor GATA-3 controls cell fate and maintenance of type 2 innate lymphoid cells.. Immunity. 2012; 37(4):634-48. (Biology). 查看参考
  2. Terashima A, Watarai H, Inoue S, et al. A novel subset of mouse NKT cells bearing the IL-17 receptor B responds to IL-25 and contributes to airway hyperreactivity.. J Exp Med. 2008; 205(12):2727-33. (Biology). 查看参考
  3. Wu L, Zepp JA, Qian W, et al. A novel IL-25 signaling pathway through STAT5.. J Immunol. 2015; 194(9):4528-34. (Biology). 查看参考
  4. Zepp JA, Wu L, Qian W, et al. TRAF4-SMURF2-mediated DAZAP2 degradation is critical for IL-25 signaling and allergic airway inflammation.. J Immunol. 2015; 194(6):2826-37. (Biology). 查看参考
  5. Zhang B, Liu C, Qian W, Han Y, Li X, Deng J. Crystal structure of IL-17 receptor B SEFIR domain.. J Immunol. 2013; 190(5):2320-6. (Biology). 查看参考
查看所有文件 (5) 查看更少内容
743815 Rev. 2

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.