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      Biotin Mouse Anti-Mouse IgM[b]
      商品详情
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      BD Pharmingen™
      Igh-6b
      Mouse (QC Testing)
      Mouse BALB/c IgG1, κ
      C57BL/10 mouse splenocytes
      ELISA (Routinely Tested), Flow cytometry (Tested During Development)
      0.5 mg/ml
      AB_394900
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553519 Rev. 11
      抗体详情
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      AF6-78

      The AF6-78 antibody reacts specifically with mouse IgM of Igh-C[b] and related haplotypes (e.g., C57BL/6, C57BL/10, SJL, AKR, NZB). It does not react with IgM of Igh-C[a] or related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2, PL, RIII). Cross-reaction with IgM of Igh-C[e] haplotype (e.g., A/J) has been observed. AF6-78 antibody does not react with free µ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation.

      553519 Rev. 11
      格式详情
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      553519 Rev.11
      报价单和参考
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      研发参考 (2)

      1. Stall AM, Loken MR. Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies. J Immunol. 1984; 132(2):787-795. (Immunogen). 查看参考
      2. Stall AM. Mouse immunoglobulin allotypes. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology. Blackwell Science Publishers; 1996:27.1-27.16.
      553519 Rev. 11

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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