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      Biotin Mouse IgG1 κ Isotype Control
      商品详情
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      BD Pharmingen™
      Mouse BALB/c IgG1, κ
      Flow cytometry, Isotype control (Routinely Tested)
      0.5 mg/ml
      AB_10050442
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      推荐的实验流程

      An isotype control should be used at the same concentration as the antibody of interest. We recommend biotinylated mouse IgG1, κ mAb MOPC-21 (Cat. no. 555747) for immunofluorescent staining of human whole blood.  The purified version of MOPC-3 is useful as a standard in ELISA (Cat. No. 557273).

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      550615 Rev. 6
      抗体详情
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      MOPC-31C

      The MOPC-31C antibody has unknown specificity. The transplantable plasmacytoma MOPC-31C was induced by intraperitoneal injection of mineral oils into BALB/c mice. It was adapted to continuous cell culture by alternate passage in animals.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      550615 Rev. 6
      格式详情
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      550615 Rev.6
      报价单和参考
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      研发参考 (2)

      1. Hay R, Caputo J, Chen TR, Macy M, McClintock P, Reid Y, ed. ATCC. Cell Lines and Hybridomas, Eighth Edition. 1994:75.
      2. Sibinovic KH, Potter M, Hoostelaere, Rode B, Wax J, ed. Catalogue of plasmacytomas and other tumors of the lymphoreticular system, 3rd edition. Kensington, Maryland: Litton Bionetics, Inc; 1976:1-33.
      550615 Rev. 6

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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