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蛋白质印迹试剂
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Single-Cell Multiomics Reagents
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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配套商品
The 2E9 antibody reacts with CD34, an ~110 kDa glycoprotein on the surface of bone marrow-derived progenitors of hematopoietic and endothelial cells. In the bone marrow, 1-3% of cells are stained with 2E9 mAb; whereas peripheral blood leukocytes are not stained. Immunomagnetic depletion of lineage-committed leukocytes from bone marrow results in about three-fold enrichment of CD34+ cells. CD34+ hematopoietic progenitors may be mobilized to the peripheral blood treatment with recombinant canine granulocyte colony-stimulating factor and stem-cell factor. Furthermore, CD34 is expressed on some canine leukemias. In the mouse, CD34 is also expressed on high endothelial venules (HEV) of lymph nodes and, in this form, functions as a ligand for L-selectin. CD34 expression on HEV of dog lymph nodes has been demonstrated with polyclonal anti-CD34 antibody.
研发参考 (10)
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Baumheter S, Singer MS, Henzel W, et al. Binding of L-selectin to the vascular sialomucin CD34. Science. 1993; 262(5132):436-438. (Biology). 查看参考
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Bhattacharya V, McSweeney PA, Shi Q, et al. Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells. Blood. 2000; 95(2):581-585. (Biology). 查看参考
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Bruno B, Nash RA, Wallace PM, et al. CD34+ selected bone marrow grafts are radioprotective and establish mixed chimerism in dogs given high dose total body irradiation. Transplantation. 1999; 68(3):338-344. (Clone-specific). 查看参考
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Georges GE, Storb R, Bruno B, et al. Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes. Blood. 2001; 98(12):3447-3455. (Biology). 查看参考
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Goerner M, Horn PA, Peterson L, et al. Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors. Blood. 2001; 98(7):2065-2070. (Clone-specific). 查看参考
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Hagglund HG, McSweeney PA, Mathioudakis G, et al. Ex vivo expansion of canine dendritic cells from CD34+ bone marrow progenitor cells. Transplantation. 2000; 70(10):1437-1442. (Clone-specific). 查看参考
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McSweeney PA, Rouleau KA, Storb R, et al. Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein. Blood. 1996; 88(6):1992-2003. (Biology). 查看参考
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McSweeney PA, Rouleau KA, Wallace PM, et al. Characterization of monoclonal antibodies that recognize canine CD34. Blood. 1998; 91(6):1977-1986. (Immunogen). 查看参考
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Niemeyer GP, Hudson J, Bridgman R, Spano J, Nash RA, Lothrop CD. Isolation and characterization of canine hematopoietic progenitor cells. Exp Hematol. 2001; 29(6):686-693. (Biology). 查看参考
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Vernau W, Moore PF. An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction. Vet Immunol Immunopathol. 1999; 69(2-4):145-164. (Biology). 查看参考
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