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      BB700 Mouse Anti-Mouse Ly-108
      商品详情
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      BD OptiBuild™
      CD352; Slamf6; SLAM family member 6; KAL1; NTB-A; SF2000
      Mouse (Tested in Development)
      Mouse IgG2a, κ
      WT thymocytes
      Flow cytometry (Qualified)
      0.2 mg/ml
      30925
      AB_2871448
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BB700 under optimal conditions that minimize unconjugated dye and antibody.
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      推荐的实验流程

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794 or 566349).

      When setting up compensation, it is recommended to compare spillover values obtained from cells and BD™ CompBeads to ensure that beads will provide sufficiently accurate spillover values.

      For optimal results, it is recommended to perform two washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescent staining prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.

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      商品通知

      1. This antibody was developed for use in flow cytometry.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Researchers should determine the optimal concentration of this reagent for their individual applications.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      9. BD Horizon Brilliant Blue 700 is covered by one or more of the following US patents: 8,455,613 and 8,575,303.
      10. Cy is a trademark of GE Healthcare.
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      742272 Rev. 1
      抗体详情
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      13G3

      The 13G3 monoclonal antibody specifically binds to Lymphocyte antigen 108, Ly-108. Ly-108 is a member of the signaling lymphocytic activation molecule (SLAM) family of immune receptors. Ly-108 is a type 1 transmembrane glycoprotein adhesion receptor that is expressed by T cells, NKT cells, B cells, NK cells, macrophages, dendritic cells and neutrophils. Ly-108 plays multiple roles in innate and adaptive immunity including costimulation of NK cell cytotoxicity and T cell cytokine responses. Moreover, Ly-108 has been implicated in autoimmunity.

      The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes.   It is a polymer-based tandem dye developed exclusively by BD Biosciences.  With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40-nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.

      742272 Rev. 1
      格式详情
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      BB700
      The BD Horizon Brilliant™ Blue 700 (BB700) Dye is part of the BD Horizon Brilliant™ Blue family of dyes. This tandem fluorochrome is comprised of a polymer-technology dye donor with an excitation maximum (Ex Max) of 476-nm and an acceptor dye with an emission maximum (Em Max) at 695-nm. Driven by BD innovation, BB700 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 695-nm (e.g., a 695/20-nm bandpass filter). The donor dye can be excited by the Violet (405 nm) laser and the acceptor dye can be excited by the red (627–640 nm) laser resulting in cross-laser excitation and fluorescence spillover. BB700 Reagents are significantly brighter than equivalent PerCP or PerCP-Cy5.5 reagents and are less sensitive to photobleaching. In addition, BB700 shows much less excitation by the violet (407-nm) laser resulting in less spillover. BB700 has minimal yellow green (562-nm) excitation and is ideal for instruments with both blue (488-nm) and yellow green (562-nm) lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BB700
      Blue 488 nm
      476 nm
      695 nm
      742272 Rev.1
      报价单和参考
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      View product citations for antibody "742272" on CiteAb

      研发参考 (5)

      1. Griewank K, C Borowski, Rietdijk S, et al. Homotypic interactions mediated by Slamf1 and Slamf6 receptors control NKT cell lineage development. Immunity. 2007; 27(5):751-762. (Clone-specific: Flow cytometry, Immunofluorescence). 查看参考
      2. Howie D, Laroux FS, Morra M, et al. Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. J Immunol. 2005; 10(174):5931-5935. (Biology). 查看参考
      3. Keszei M, Detre C, Rietdijk ST, et al. A novel isoform of the Ly108 gene ameliorates murine lupus.. J Exp Med. 2011; 208(4):811-22. (Immunogen: ELISA, Flow cytometry, Immunoprecipitation). 查看参考
      4. Li W, Sofi MH, Rietdijk S, Wang N, et al. The SLAM-Associated Protein (SAP)/Fyn/PKCθ Pathway is Required for Thymocyte-mediated CD4 T Cell Development. Immunity. 2007; 27(2):763-774. (Biology). 查看参考
      5. Peck SR, Ruley HE. Ly108: a new member of the mouse CD2 family of cell surface proteins. J Immunol. 2000; 52(1-2):63-72. (Biology). 查看参考
      查看所有文件 (5) 查看更少内容
      742272 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.