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Two-color flow cytometric analysis of S100A9 (MRP-14) expression in mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse Ly-6G and LY-6C antibody (Cat. No. 553128/561084). The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714) and stained with either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Cat. No. 557731; Left Plot) or Alexa Fluor® 647 Rat Anti-Mouse S100A9 (MRP-14) antibody (Cat. No. 565833; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of S100A9 (MRP-14) [or Ig Isotype control staining] versus Ly-6G and LY-6C were derived from gated events with the forward and side light-scatter characteristics of intact bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System.
BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Mouse S100A9 (MRP-14)
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
配套商品
The 2B10 monoclonal antibody specifically binds to S100 calcium-binding protein A9 (S100A9), which is also known as Migration inhibitory factor-related protein 14 (MRP-14) or Calgranulin-B (CAGB). S100A9 is a member of the S100 family of calcium and zinc binding proteins. S100A9 is predominantly expressed in myeloid cells, such as, neutrophils and monocytes. It is one of the most abundant proteins expressed in myeloid cells. Similar to other S100 family of proteins, S100A9 functions as a calcium sensor and may enhance the chemotaxis and proinflammatory response of monocytes and neutrophils.
研发参考 (4)
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Baker JR1, Jeffery R, May RD, et al. Dev Dyn. 2011; 240(9):2194-2203. (Clone-specific: Western blot). 查看参考
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Foell D, Wittkowski H, Vogl T, Roth J. S100 proteins expressed in phagocytes: a novel group of damage-associated molecular pattern molecules.. J Leukoc Biol. 2007; 81(1):28-37. (Biology). 查看参考
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Hobbs JA, May R, Tanousis K, et al. Myeloid cell function in MRP-14 (S100A9) null mice.. Mol Cell Biol. 2003; 23(7):2564-76. (Immunogen: Flow cytometry, Immunohistochemistry, Western blot). 查看参考
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Nacken W, Roth J, Sorg C, Kerkhoff C. S100A9/S100A8: Myeloid representatives of the S100 protein family as prominent players in innate immunity.. Microsc Res Tech. 2003; 60(6):569-80. (Biology). 查看参考
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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