-
Reagents
- Flow Cytometry Reagents
-
蛋白质印迹试剂
- 免疫分析 试剂
-
Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
-
Functional Assays
-
显微成像试剂
-
Cell Preparation and Separation Reagents
-
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
- China (Chinese)
- 更改国家/语言
Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
BD Pharmingen™ Alexa Fluor® 647 Rat anti-Mouse CD371 (Clec12A)
克隆 5D3/CD371 (also known as 1/06-5D3)
(RUO)Multicolor flow cytometric analysis of CD371 (Clec12A) expression on mouse spleen dendritic cell subsets. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827), PerCP-Cy™5.5 Hamster Anti-Mouse CD11c (Cat. No. 560584), APC-H7 Rat Anti-Mouse CD19 (Cat. No. 560143/560245), PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD49b (Cat. No. 562453) and either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; dashed line histogram) or Alexa Fluor® 647 Rat Anti-Mouse Clec12A (Cat. No. 564794; solid line histogram). Overlapping histograms showing Clec12A expression (or Ig Isotype control staining) on either CD11c-high CD45R/B220- (Left Panel) or CD11c-intermediate CD45R/B220+ (Right Panel) cells were derived from gated events with the forward and side light-scattering characteristics of viable CD3- CD19- CD49b- leucocytes. Flow cytometric analysis was performed on a BD™ LSR II.
BD Pharmingen™ Alexa Fluor® 647 Rat anti-Mouse CD371 (Clec12A)
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The 5D3/CD371 (aka, 1/06-5D3) monoclonal antibody specifically binds to mouse CD371 which is encoded by Clec12A (C-type lectin domain family 12 member A). CD371 is also known as Myeloid inhibitory C-type lectin-like receptor (MICL), C-type lectin-like molecule 1 (CLL-1) or Dendritic cell-associated lectin 2 (DCAL-2). CD371 is a ~30 kDa type II transmembrane glycoprotein that possesses one extracellular C-type lectin domain and one cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). CD371 is expressed on plasmacytoid dendritic cells, subsets of myeloid dendritic cells, monocytes, macrophages and B cells. It reportedly serves as a negative regulator of granulocyte and monocyte function.
研发参考 (4)
-
Lahoud MH, Proietto AI, Ahmet F, et al. The C-type lectin Clec12A present on mouse and human dendritic cells can serve as a target for antigen delivery and enhancement of antibody responses.. J Immunol. 2009; 182(12):7587-94. (Immunogen: Flow cytometry, Fluorescence microscopy, Functional assay, Immunofluorescence, In vivo exacerbation, Stimulation). 查看参考
-
Marshall AS, Willment JA, Lin HH, Williams DL, Gordon S, Brown GD. Identification and characterization of a novel human myeloid inhibitory C-type lectin-like receptor (MICL) that is predominantly expressed on granulocytes and monocytes.. J Biol Chem. 2004; 279(15):14792-802. (Biology). 查看参考
-
Marshall AS, Willment JA, Pyz E, et al. Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation.. Eur J Immunol. 2006; 36(8):2159-69. (Biology). 查看参考
-
van Rhenen A, van Dongen GA, Kelder A, et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination between normal and leukemic stem cells.. Blood. 2007; 110(7):2659-66. (Biology). 查看参考
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.