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Purified Mouse Anti-SATB1
Purified Mouse Anti-SATB1
Western blot analysis of SATB1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1: 500, lane 3: 1: 1000 dilution of the mouse anti- SATB1 antibody.
Western blot analysis of SATB1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1: 500, lane 3: 1: 1000 dilution of the mouse anti- SATB1 antibody.
商品详情
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BD Transduction Laboratories™
SATB homeobox 1; Special AT-rich sequence-binding protein 1
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human SATB1 aa. 550-667
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
106 kDa
250 µg/ml
AB_398716
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611182 Rev. 2
抗体详情
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14/SATB1

The 14/SATB1 monoclonal antibody specifically binds to the special AT-rich sequence binding protein 1 that is encoded by the SATB1 (SATB homeobox 1) gene. Central to the formation of nucleosomes, which consist of octameric histone cores with defined segments of chromatin wound around them, are the interactions of nucleosomes with nuclear matrix components. Specific genomic DNA segments that interact with the nuclear matrix are called scaffold or matrix attachment regions (SARs or MARs). SATB1 is a homeodomain MAR binding protein. It recognizes ATC sequences that consist of stretches of A's, T's, and C's on one DNA strand. SATB1 is expressed by thymocytes (especially single positive CD4+ thymocytes), T and B lymphocytes, NK cells, and macrophages. SATB1 can recruit chromatin-remodeling factors and thereby regulate chromatin structure and gene expression. Recently it has been reported that repression of SATB1 was essential for the phenotype and function of mouse regulatory T cells but inhibitory for the establishment of transcriptional programs expressed by effector T cells.

611182 Rev. 2
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611182 Rev.2
报价单和参考
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研发参考 (6)

  1. Alvarez JD, Yasui DH, Niida H, Joh T, Loh DY, Kohwi-Shigematsu T. The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development. Genes Dev. 2000; 14(5):521-535. (Biology). 查看参考
  2. Beyer M, Thabet Y, Müller R-U, et al. Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. Nat Immunol. 2011; 12:898-907. (Clone-specific: Flow cytometry, Western blot). 查看参考
  3. Cai S, Kohwi-Shigematsu T.. Intranuclear relocalization of matrix binding sites during T cell activation detected by amplified fluorescence in situ hybridization.. 1999; 19(3):394-402. (Biology). 查看参考
  4. Dickinson LA, Joh T, Kohwi Y, Kohwi-Shigematsu T. A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition. Cell. 1992; 70(4):631-645. (Biology). 查看参考
  5. Liu J, Bramblett D, Zhu Q. The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression. Mol Cell Biol. 1997; 17(9):5275-5287. (Biology). 查看参考
  6. de Belle I, Cai S, Kohwi-Shigematsu T. The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops. J Cell Biol. 1998; 141(2):335-348. (Biology). 查看参考
查看所有文件 (6) 查看更少内容
611182 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.