准备和存储
推荐的实验流程
Applications include western blot analysis (1-2 µg/ml). Human cell lines including Jurkat T cells (ATCC TIB-152), 293 adenovirus-transformed kidney cells (ATCC CRL-1573) and Daudi Burkitt lymphoma cells (ATCC CCL-213) are recommended as positive controls.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
TRAFs (TNF receptor associated factors) are signal transducing molecules which participate in forming TNF/Nerve Growth Factor receptor-associated signalling complexes. TRAF proteins share a novel C-terminal homology region, the "TRAF" domain, a coiled-coiled region and the majority have Zinc/RING finger motifs. At least six TRAF proteins (TRAFs 1-6) have been identified. These proteins are cytoplasmic adapters; they bind to cytoplasmic domains of various receptors and can function to recruit other proteins to a signaling complex thereby promoting intracellular signal transduction. Specifically, TRAF2 has been shown to interact with the following surface receptors: TNFRII, CD27, CD30, CD40, 4-1BB, Ox40, HVEM/ATAR and LMP-1. TRAF2 has also been shown to associate with intracellular proteins, including TRADD, FADD, I-TRAF/TANK, TRIP, A20, c-IAP1 and 2, Casper, RIP and NIK. Thus, the interaction of TRAF2 with these various receptors and intracellular proteins plays an important role modulating downstream events which can result in inducing cell death or keeping cells alive. For example, cells deficient in TRAF2 demonstrated increased sensitivity to TNF-induced cell death, but a decrease in TNF-induced JNK activation, and little change on NF-kB activation. TRAF2 is detected at ~ 53 kDa in western blot analysis. The C90-481 antibody reacts with human TRAF2. A synthetic protein freagment corresponding to amino acids 93-199 of human TRAF2 was used as immunogen.
研发参考 (6)
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Arch RH, Gedrich RW, Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs)--a family of adapter proteins that regulates life and death. Genes Dev. 1998; 12(18):2821-2830. (Biology). 查看参考
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Hsu H, Shu HB, Pan MG, Goeddel DV. TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways. Cell. 1996; 84(2):299-308. (Biology). 查看参考
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Rothe M, Sarma V, Dixit VM, Goeddel DV. TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40. Science. 1995; 269(5229):1424-1427. (Biology). 查看参考
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Shu HB, Takeuchi M, Goeddel DV. The tumor necrosis factor receptor 2 signal transducers TRAF2 and c-IAP1 are components of the tumor necrosis factor receptor 1 signaling complex. Proc Natl Acad Sci U S A. 1996; 93(24):13973-13978. (Clone-specific: Western blot). 查看参考
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Tsitsikov EN, Wright DA, Geha RS. CD30 induction of human immunodeficiency virus gene transcription is mediated by TRAF2. Proc Natl Acad Sci U S A. 1997; 94(4):1390-1395. (Biology). 查看参考
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Yeh WC, Shahinian A, Speiser D. Early lethality, functional NF-kappaB activation, and increased sensitivity to TNF-induced cell death in TRAF2-deficient mice. Immunity. 1997; 7(5):715-725. (Clone-specific: Activation). 查看参考
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