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Purified Mouse Anti-Human PDGF-AB/BB
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1
Human PDGF BB Recombinant Protein
Western blot (Routinely Tested), Immunoprecipitation (Reported)
35-56 kDa
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.


Western blot:  Please refer to

Note: Investigators should note that due to the ability for this antibody to recognize various PDGF isoforms and/or multimers, bands may be observable migrating in a range between 35-56 kDa (e.g 35 kDa, 42 kDa, and/or 56 kDa). In WI-38 cells (Human lung fibroblasts; ATCC CCL-75), for example, a band may be predominantly observable to migrate ~ 50-56 kDa.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to for technical protocols.
554284 Rev. 10
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Platelet derived growth factor (PDGF) plays a role in regulating a number of biological processes including cellular proliferation and development. PDGF consists of a disulfide-linked homo- or heterodimer of two chains, A and B, which are encoded by two separate genes. The three possible combinations of the PDGF chains, AA, AB, and BB, are all biologically active. The PDGF receptor (PDGFR) is a protein tyrosine kinase. Ligand binding to the PDGFR leads to tyrosine phosphorylation of numerous intracellular proteins, including the receptor itself. The PDGFR consists of two subunits, α and β, which are encoded by two separate genes. The α subunit binds to both the A and B PDGF chains with high affinity, whereas the β subunit binds only to the B chain with high affinity. Ligand binding results in receptor dimerization, with the PDGF type (AA, AB or BB) influencing the resulting PDGFR subunit composition (αα, αβ, or ββ). That is, PDGF-AA binds to αα receptors, PDGF-AB binds to αα and αβ receptors, and PDGF-BB binds to αα, αβ, and ββ receptors. This antibody reportedly recognizes B chain-containing human PDGF isoforms and does not recognize the PDGF-AA homodimer. Additionally, this antibody reportedly inhibits PDGFR binding of PDGF-AB and -BB, but not PDGF-AA and is thought to recognize a conformational epitope of the PDGF B chain that is dependent on disulfide linkages. This antibody has been reported to inhibit PDGF receptor-binding and mitogenic effects of PDGF-AB and -BB.

554284 Rev. 10
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554284 Rev.10
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View product citations for antibody "554284" on CiteAb

研发参考 (2)

  1. Bazenet CE, Kazlauskas A. The PDGF receptor alpha subunit activates p21ras and triggers DNA synthesis without interacting with rasGAP. Oncogene. 1993; 9(2):517-525. (Biology). 查看参考
  2. LaRochelle WJ, Robbins KC, Aaronson SA. Immunochemical localization of the epitope for a monoclonal antibody that neutralizes human platelet-derived growth factor mitogenic activity. Mol Cell Biol. 1989; 9(8):3538-3542. (Biology: Blocking, Immunoprecipitation, Neutralization, Western blot). 查看参考
554284 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.