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Purified Mouse Anti-Human IKKβ w/ Control

BD Pharmingen™ Purified Mouse Anti-Human IKKβ w/ Control

Purified Mouse Anti-Human IKKβ w/ Control
Western blot analysis of Ikkβ. Lysate from HeLa cells was probed with anti-Ikkβ (clone F18-1875, Cat. No. 51-8120GR) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Ikkβ is identified as a band of ~87 kDa.
Western blot analysis of Ikkβ. Lysate from HeLa cells was probed with anti-Ikkβ (clone F18-1875, Cat. No. 51-8120GR) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Ikkβ is identified as a band of ~87 kDa.
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BD Pharmingen™
Human (QC Testing)
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)


Applications include western blot analysis (0.06 - 0.25 µg/ml). Other applications not routinely tested at BD Biosciences Pharmingen include immunoprecipitation (4 µg/sample). Store the antibody at -20°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a positive control (Cat. No. 51-16516N; store lysate at -20°C). HeLa control lysate (Cat. No. 611449) is sold separately as a ready-to-use western blot control.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
551920 Rev. 7
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描述 数量/尺寸 零件号 EntrezGene ID
Purified Mouse Anti-Human IKKβ 50 µg (1 ea) 51-8120GR N/A
HeLa Control Lysate 50 µg (1 ea) 51-16516N N/A
551920 Rev. 7
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研发参考 (11)

  1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature. 1997; 388(6642):548-554. (Biology). 查看参考
  2. Israël A. The IKK complex: an integrator of all signals that activate NF-kappaB. Trends Cell Biol. 2000; 10(4):129-133. (Biology). 查看参考
  3. Lee FS, Hagler J, Chen ZJ, Maniatis T. Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway. Cell. 1997; 88(2):213-222. (Biology). 查看参考
  4. Li Q, Van Antwerp D, Mercurio F, Lee KF, Verma IM. Severe liver degeneration in mice lacking the IkappaB kinase 2 gene. Science. 1999; 284(5412):321-325. (Biology). 查看参考
  5. Li ZW, Chu W, Hu Y, et al. The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis. J Exp Med. 1999; 189(11):1839-1845. (Biology). 查看参考
  6. Ling L, Cao Z, Goeddel DV. NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci U S A. 1998; 95(7):3792-3797. (Biology). 查看参考
  7. Malinin NL, Boldin MP, Kovalenko AV, Wallach D. MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1. Nature. 1997; 385(6616):540-544. (Biology). 查看参考
  8. Mercurio F, Zhu H, Murray BW, et al. IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science. 1997; 278(5339):860-866. (Biology). 查看参考
  9. Nakano H, Shindo M, Sakon S, et al. Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl Acad Sci U S A. 1998; 95(7):3537-3542. (Biology). 查看参考
  10. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature. 1998; 395(6699):297-300. (Biology). 查看参考
  11. Tanaka M, Fuentes ME, Yamaguchi K, et al. Embryonic lethality, liver degeneration, and impaired NF-kappa B activation in IKK-beta-deficient mice. Immunity. 1999; 10(4):421-429. (Biology). 查看参考
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551920 Rev. 7

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.