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      Purified Mouse Anti-HPV-16 L1
      Purified Mouse Anti-HPV-16 L1
      Immunohistochemistry for HPV type 16.  A formalin-fixed, paraffin-embedded section from human cervix infected with HPV type 16 was stained with the Purified Mouse Anti-HPV 16 L1 antibody. Cells expressing the L1 protein of the HPV 16 virus can be identifed by the intense brown labeling (magnification 20X).
      Purified Mouse Anti-HPV-16 L1
      Immunohistochemistry for HPV type 16.  A formalin-fixed, paraffin-embedded section from human cervix infected with HPV type 16 was stained with the Purified Mouse Anti-HPV 16 L1 antibody. Cells expressing the L1 protein of the HPV 16 virus can be identifed by the intense brown labeling (magnification 20X).
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG2a, κ
      Immunohistochemistry-formalin (antigen retrieval required) (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
      AB_393919
      Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Immunohistochemistry:  For optimal indirect immunohistochemical staining, this antibody should be titrated, such as a 1:10 to 1:50 dilution, and visualized via a three-step staining procedure in combination with polyclonal Biotin Goat Anti-Mouse Ig (Cat. No. 550337) as the secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880).  Alternatively, investigators may be interested in the BD Pharmingen™ Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011).

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      550840 Rev. 2
      抗体详情
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      CAMVIR-1

      More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion.  Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres.  HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33.

      550840 Rev. 2
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550840 Rev.2
      报价单和参考
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      研发参考 (4)

      1. Doorbar J, Ely S, Coleman N, Hibma M, Davies DH, Crawford L. Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein. Virology. 1992; 187(1):353-359. (Biology). 查看参考
      2. McLean CS, Churcher MJ, Meinke J. Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. J Clin Pathol. 1990; 43(6):488-492. (Biology). 查看参考
      3. Zhou J, Sun XY, Stenzel DJ, Frazer IH. Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles. Virology. 1991; 185(1):251-257. (Biology). 查看参考
      4. de Villiers EM. Heterogeneity of the human papillomavirus group. J Virol. 1989; 63(11):4898-4903. (Biology). 查看参考
      查看所有文件 (4) 查看更少内容
      550840 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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