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Western blot analysis of TRADD on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-TRADD antibody.
Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).
BD Transduction Laboratories™ Purified Mouse Anti-TRADD
BD Transduction Laboratories™ Purified Mouse Anti-TRADD
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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TNFα (Tumor Necrosis Factor) stimulates programmed cell death and NF-κB activation as a result of its binding to the TNF receptor 1 (TNFR1). Within this receptor, a sequence referred to as the "death domain" has been shown to be necessary for both of these functions. Using the yeast two-hybrid system to detect proteins which interact with the receptor through this "death domain", a 34 kDa protein was found and designated TRADD (TNFR1-Associated Death Domain protein). TRADD appears to contain no intrinsic catalytic activity. It also contains a death domain and it has been shown to bind to FADD and RIP. Mutational analysis of TRADD demonstrates that programmed cell death and NF-κB activation are distinct and controlled independently.
研发参考 (5)
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He KL, Ting AT. A20 inhibits tumor necrosis factor (TNF) alpha-induced apoptosis by disrupting recruitment of TRADD and RIP to the TNF receptor 1 complex in Jurkat T cells. Mol Cell Biol. 2002; 22(17):6034-6045. (Biology: Western blot). 查看参考
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Hsu H, Xiong J, Goeddel DV. The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation. Cell. 1995; 81(4):495-504. (Biology). 查看参考
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Morgan M, Thorburn J, Pandolfi PP, Thorburn A. Nuclear and cytoplasmic shuttling of TRADD induces apoptosis via different mechanisms. J Cell Biol. 2002; 157(156):975-984. (Biology: Immunofluorescence). 查看参考
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Park A, Baichwal VR. Systematic mutational analysis of the death domain of the tumor necrosis factor receptor 1-associated protein TRADD. J Biol Chem. 1996; 271(16):9858-9862. (Biology). 查看参考
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Yount GL, Afshar G, Ries S. Transcriptional activation of TRADD mediates p53-independent radiation-induced apoptosis of glioma cells. Oncogene. 2001; 20(22):2826-2835. (Biology: Western blot). 查看参考
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