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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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Members of the Protein Kinase C (PKC) family of homologous serine/threonine protein kinases are involved in a number of processes such as cell growth, cell differentiation, and cytokine secretion. PKCs are activated by Ca2+, phospholipids, diacylglycerol, phorbol esters, and proteolysis. PRK1 (PKC-Related Kinase 1, also named PKN) was originally identified in human hippocampus as a novel protein kinase with sequence homology to PKC. PRK1 contains 942 amino acids with an apparent molecular weight of 120 kDa. Although activated by limited proteolysis, PRK1 is not activated by Ca2+/diacylglycerol or phorbol esters. However, PRK1 is activated by phospholipids and arachidonic acid. PRK1 may regulate cytoskeletal changes since it binds to Rho-GTP and becomes phosphorylated in vivo, coincidentally with the formation of focal adhesions and stress fibers.
研发参考 (5)
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Flynn P, Mellor H, Casamassima A, Parker PJ. Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase. J Biol Chem. 2000; 275(15):11064-11070. (Clone-specific: Western blot). 查看参考
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Hughes WE, Larijani B, Parker PJ. Detecting protein-phospholipid interactions. Epidermal growth factor-induced activation of phospholipase D1b in situ. J Biol Chem. 2002; 277(25):22974-22979. (Biology: Western blot). 查看参考
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Mukai H, Kitagawa M, Shibata H. Activation of PKN, a novel 120-kDa protein kinase with leucine zipper-like sequences, by unsaturated fatty acids and by limited proteolysis. Biochem Biophys Res Commun. 1994; 204(1):348-356. (Biology). 查看参考
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Mukai H, Kitagawa M, Shibata H. Activation of PKN, a novel 120-kDa protein kinase with leucine zipper-like sequences, by unsaturated fatty acids and by limited proteolysis. Biochem Biophys Res Commun. 1994; 204(1):348-356. (Biology). 查看参考
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Palmer RH, Ridden J, Parker PJ. Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family. Eur J Biochem. 1995; 227(1-2):344-351. (Biology). 查看参考
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