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Western blot analysis of PMCA2 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-PMCA2 antibody.
Immunofluorescence staining of SK-BR-3 cells (Human breast adenocarcinoma; ATCC HTB-30).
BD Transduction Laboratories™ Purified Mouse Anti-PMCA2
BD Transduction Laboratories™ Purified Mouse Anti-PMCA2
监管状态图例
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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Depolarization of the plasma membrane during neurotransmitter release or muscle excitation/contraction involves increases in intracellular Ca2+. Homeostasis is reestablished through the action of various enzymes, including ion pumps. PMCAs (Plasma Membrane Calcium ATPases) belong to the P-type family of transport ATPases which couple ATP hydrolysis to the export of intracellular Ca2+. They are characterized by large molecular mass, high affinity for Ca2+, and direct regulation by interaction with Ca2+/calmodulin. Four different genes (PMCA 1-4) and independent alternative splice sites combine to generate multiple PMCA isoforms. PMCA 1-4 are highly homologous, but differ substantially in their N-terminal regions. PMCA1 and 4 are ubiquitously expressed and perform housekeeping roles. However, PMCA2 and 3 exhibit tissue-specific expression and perform more specialized functions. Although PMCA2 and 3 are both expressed to some degree in muscle, PMCA2 is primarily found in the cerebellum. There, it accounts for the majority of neural pump protein. Thus, PMCA2 is thought to be the principal ATPase that functions to maintain Ca2+ homeostasis in response to neural excitation.
研发参考 (4)
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Filoteo AG, Elwess NL, Enyedi A, Caride A, Aung HH, Penniston JT. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem. 1997; 272(38):23741-23747. (Biology). 查看参考
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Hilfiker H, Guerini D, Carafoli E. Cloning and expression of isoform 2 of the human plasma membrane Ca2+ ATPase. Functional properties of the enzyme and its splicing products. J Biol Chem. 1994; 269(42):26178-26183. (Biology). 查看参考
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Shull GE, Greeb J. Molecular cloning of two isoforms of the plasma membrane Ca2+-transporting ATPase from rat brain. Structural and functional domains exhibit similarity to Na+,K+- and other cation transport ATPases. J Biol Chem. 1988; 263(18):8646-8657. (Biology). 查看参考
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Stauffer TP, Hilfiker H, Carafoli E, Strehler EE. Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes. J Biol Chem. 1993; 268(34):25993-26003. (Biology). 查看参考
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