BD Transduction Laboratories™ Purified Mouse Anti-PI3 Kinase p110δ

Phosphoinositide 3 Kinase (PI3 Kinase) phosphorylates the D-3 position of the inositol ring of phosphatidylinositol, producing PtdIns(3)P, PtdIns(3,4)P2, and PtdIns (3,4,5)P3. PI3-kinase is a heterodimer of an 85 kDa regulatory subunit (p85) containing two SH2 domains and an SH3 domain, and a 110 kDa catalytic subunit (p110). Four isoforms of p110 have been identified (α , β , γ, and δ). The p110α, β isoforms are distributed ubiquitously. The p110δ isoform is found exclusively in lymphocytes and lymphoid tissues. Lymphocytes also contains p110α and both isoforms interact with p85 and are recruited to activated signaling complexes. However, several biochemical and structural differences distinguish p110δ. Unlike p110α, p110δ autophosphorylates and does not phosphorylate p85. In addition, p110δ contains a Pro-rich region, which endows it with the potential for interaction with SH3 domain-containing proteins, and a bZIP-like domain, which is a common transcription factor domain that has also been identified in certain protein kinases. With the established role of PI3-Ks in cytoskeletal rearrangements, it is thought that p110δ contributes specifically to the regulation of lymphocytes extravasation into tissues. There is a high degree of homology in the immunogen region with the P110β form (77%), and it is likely that our antibody will detect both the beta and delta forms.