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Western blot analysis of Lyn on HEL (human erythroleukemia cell line) lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Lyn.
Immunofluorescent staining on Human Fibroblasts
BD Transduction Laboratories™ Purified Mouse Anti-Lyn
BD Transduction Laboratories™ Purified Mouse Anti-Lyn
监管状态图例
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准备和存储
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Lyn is a 56 kDa cytosolic tyrosine kinase that belongs to the Src family of kinases. In addition to Src, this family includes lck, Fgr, Hck, Blk, Yes, fyn, and Yrk kinases. The lyn gene was isolated from a human placenta cDNA library and later found in a variety of cell types. Similar to other members of the Src family of tyrosine kinases, Lyn has been implicated in the regulation of cell signaling mechanisms. Lyn's function has primarily been studied in hematopoietic cells where it is associated with the high-affinity IgE receptor in basophils. It also associates with p120 Ras-GAP in thrombin-stimulated platelets. This association corresponds to an increase in Lyn kinase activity. Lyn is often observed as a doublet of about 56 kDa and 53 kDa. These forms may represent alternative splicing products of lyn mRNA.
研发参考 (5)
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Bolen JB, Rowley RB, Spana C, Tsygankov AY. The Src family of tyrosine protein kinases in hemopoietic signal transduction. FASEB J. 1992; 6(15):3403-3409. (Biology). 查看参考
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Deehan MR, Harnett W, Harnett MM. A filarial nematode-secreted phosphorylcholine-containing glycoprotein uncouples the B cell antigen receptor from extracellular signal-regulated kinase-mitogen-activated protein kinase by promoting the surface Ig-mediated recruitment of Src homology 2 domain-containing tyrosine phosphatase-1 and Pac-1 mitogen-activated kinase-phosphatase. J Immunol. 2001; 166(12):7462-7468. (Clone-specific: Western blot). 查看参考
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Liang X, Wisniewski D, Strife A, Shivakrupa, Clarkson B, Resh MD. Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling. J Biol Chem. 2002; 277(16):13732-13738. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
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Liu J, Oh P, Horner T, Rogers RA, Schnitzer JE. Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains. J Biol Chem. 1997; 272(11):7211-7222. (Clone-specific). 查看参考
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Muller G, Jung C, Wied S, Welte S, Jordan H, Frick W. Redistribution of glycolipid raft domain components induces insulin-mimetic signaling in rat adipocytes. Mol Cell Biol. 2001; 21(14):4553-4567. (Clone-specific: Immunoprecipitation, Western blot). 查看参考
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