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Western blot analysis of Karyopherin α on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-karyopherin antibody.
Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).
BD Transduction Laboratories™ Purified Mouse Anti-Karyopherin α
BD Transduction Laboratories™ Purified Mouse Anti-Karyopherin α
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推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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The two step process of importing proteins into the nucleus involves the binding and interaction of several cytosolic and nuclear pore proteins. Proteins to be translocated into the nucleus contain a nuclear localization sequence (NLS) which is recognized and bound by carrier proteins in the cytosol. Heterodimers belonging to a highly conserved family of proteins called karyopherins are required for successful nuclear localization of cytosolic proteins. The α-subunits appear to function in the binding of NLS (both simple and bitartite NLS motifs), but both α- and β-subunits are required for successful docking to the nuclear envelope. ATP is required for complete translocation of proteins into the nucleus. Karyopherin α2 was first identified as Rch-1, an NLS receptor which interacts with the RAG-1 recombination-activating protein in developing B and T cells. Rch-1 has been reported to be 44% identical to karyopherin α1 (hSRP-1 /NPI-1).
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (4)
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Cuomo CA, Kirch SA, Gyuris J, Brent R, Oettinger MA. Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein. Proc Natl Acad Sci U S A. 1994; 91(13):6156-6160. (Biology). 查看参考
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Grozinger CM, Schreiber SL. Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization. Proc Natl Acad Sci U S A. 2000; 97(14):7835-7840. (Biology: Western blot). 查看参考
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Moroianu J, Hijikata M, Blobel G, Radu A. Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins. Proc Natl Acad Sci U S A. 1995; 92(14):6532-6536. (Biology). 查看参考
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Weis K, Mattaj IW, Lamond AI. Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences. Science. 1995; 268(5213):1049-1053. (Biology). 查看参考
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