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Western blot analysis of GS27 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-GS27 antibody.
Immunofluorescence staining of human endothelial cells.
BD Pharmingen™ Purified Mouse Anti-GS27
BD Pharmingen™ Purified Mouse Anti-GS27
监管状态图例
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准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Eukaryotic protein trafficking involves the packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Components of both the vesicle and the synaptic plasma membrane interact to form a fusion complex that mediates vesicle docking and fusion. This fusion complex contains NSF (N-ethyl-maleimide-sensitive factor), SNAPs (soluble NSF attachment proteins), and receptor proteins (SNAREs) that include synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosome-associated protein of 25 kDa). Interactions between vesicle SNAREs and target membrane SNARES mediates the specificity of docking. Along one pathway of protein trafficking, the Golgi apparatus receives proteins from the ER. These proteins move from cis-Golgi to trans-Golgi, through a stack of cisternae, towards the trans-Golgi network. From here, they are delivered to their proper destination in the cell. GS27 (Golgi SNARE of 27 kDa) (membrin) is a Golgi-associated SNARE. It functions in medial-to-trans-Golgi protein movement.
研发参考 (3)
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Hay JC, Chao DS, Kuo CS, Scheller RH. Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells. Cell. 1997; 89(1):149-158. (Biology). 查看参考
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Hay JC, Klumperman J, Oorschot V, Steegmaier M, Kuo CS, Scheller RH. Localization, dynamics, and protein interactions reveal distinct roles for ER and Golgi SNAREs. J Cell Biol. 1998; 141(7):1489-1502. (Biology). 查看参考
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Lowe SL, Peter F, Subramaniam VN, Wong SH, Hong W. A SNARE involved in protein transport through the Golgi apparatus. Nature. 1997; 389(6653):881-884. (Biology). 查看参考
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