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LEFT IMAGE: Immunofluorescence staining of A431 cells. RIGHT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin (Cat. No. 610181/610182). E-Cadherin is observable at 120kDa. Left Panel: A431 lysate (ATCC CRL-1555; Human epithelial carcinoma) was blotted at 1:10000 & 1:20000 (Lanes 1 & 2 respectively; 30 second exposure). Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 (Lanes 1 & 2 respectively; 30 second exposure). Middle Right Panel: 293F cells transfected with human E-Cadherin (CDH1) was blotted at 1:2500 & 1: 5000 (Lanes 1 & 2 respectively; 5 second exposure). Right Panel: 293 cells transfected with human P-Cadherin (CDH3) was blotted using Purified Mouse Anti-E-Cadherin (Cat. No. 610181/610182) at 1:2500 & 1: 5000 (Lanes 1 & 2 respectively; 5 second exposure).
BD Transduction Laboratories™ FITC Mouse Anti- E-Cadherin
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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E-Cadherin is a 120-kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. The 36/E-Cadherin monoclonal antibody recognizes the cytoplasmic domain of E-Cadherin, regardless of phosphorylation status. The peptide immunogen was generated from human E-Cadherin aa. 735-883.
Note: Investigators are advised that this antibody has some degree of cross-reactivity to P-Cadherin.
研发参考 (7)
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Behrens J, Vakaet L, Friis R, et al. Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene.. J Cell Biol. 1993; 120(3):757-66. (Biology). 查看参考
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Jaksits S, Kriehuber E, Charbonnier AS, Rappersberger K, Stingl G, Maurer D. CD34+ cell-derived CD14+ precursor cells develop into Langerhans cells in a TGF-beta 1-dependent manner. J Immunol. 1999; 163(9):4869-4877. (Clone-specific: Flow cytometry). 查看参考
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Miyoshi K, Shillingford JM, Smith GH, et al. Signal transducer and activator of transcription (Stat) 5 controls the proliferation and differentiation of mammary alveolar epithelium. J Cell Biol. 2001; 155(4):531-542. (Clone-specific: Immunohistochemistry). 查看参考
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Sheibani N, Sorenson CM, Frazier WA. Differential modulation of cadherin-mediated cell-cell adhesion by platelet endothelial cell adhesion molecule-1 isoforms through activation of extracellular regulated kinases. Mol Biol Cell. 2000; 11(8):2793-2802. (Clone-specific: Immunofluorescence, Western blot). 查看参考
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Takeichi M. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.. Development. 1988; 102(4):639-55. (Biology). 查看参考
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Tamm I, Cardinale I, Kikuchi T, Krueger JG. E-cadherin distribution in interleukin 6-induced cell-cell separation of ductal breast carcinoma cells. Proc Natl Acad Sci U S A. 1994; 91(10):4338-4342. (Biology). 查看参考
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Weng Z, Xin M, Pablo L, et al. Protection against anoikis and down-regulation of cadherin expression by a regulatable beta-catenin protein. J Biol Chem. 2002; 277(21):18677-18686. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
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