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Purified NA/LE Rat Anti-Mouse IL-18 Receptor β
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BD Pharmingen™
Mouse (QC Testing)
Rat IgG2a, λ
Mouse sIL-1R7 fusion protein
ELISA (Routinely Tested), Neutralization (Tested During Development)
1.0 mg/ml
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.


Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to for technical protocols.
552422 Rev. 3
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The TC30-28E3 antibody specifically binds to the β subunit of the mouse Interleukin-18 Receptor complex (IL-18Rβ). The mouse IL-18R complex is expressed by T cells stimulated with IL-12 alone or cultured with antigen and IL-12 to induce Th1 cells. IL-18R is constitutively expressed by NK cells. Resting mouse T cells and Th2 cells do not express detectable levels of IL-18R. The mouse IL-18R complex consists of the IL-18α chain, also known as IL-1 Receptor related protein 1 (IL-1Rrp1 or IL-R5) and the IL-18Rβ chain also known as IL-1R accessory protein-like (IL-1RAcPL or IL-R7). The cell surface IL-18Rα chain binds IL-18 with low affinity. However its interaction with the IL-18Rβ chain confers high affinity binding for the ligand. The IL-18Rβ chain is not involved in ligand binding but is responsible for the transduction of biological signals. The immunogen used to generate the TC30-28E3 hybridoma was sIL-1R7 fusion protein.

552422 Rev. 3
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NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
552422 Rev.3
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View product citations for antibody "552422" on CiteAb

研发参考 (9)

  1. Born TL, Thomassen E, Bird TA, Sims JE. Cloning of a novel receptor subunit, AcPL, required for interleukin-18 signaling. J Biol Chem. 1998; 273(45):29445-29450. (Biology). 查看参考
  2. Debets R, Timans JC, Churakowa T, et al. IL-18 receptors, their role in ligand binding and function: anti-IL-1RAcPL antibody, a potent antagonist of IL-18. J Immunol. 2000; 165(9):4950-4956. (Clone-specific). 查看参考
  3. Hoshino K, Tsutsui H, Kawai T, et al. Cutting edge: generation of IL-18 receptor-deficient mice: evidence for IL-1 receptor-related protein as an essential IL-18 binding receptor. J Immunol. 1999; 162(9):5041-5044. (Biology). 查看参考
  4. Hyodo Y, Matsui K, Hayashi N, et al. IL-18 up-regulates perforin-mediated NK activity without increasing perforin messenger RNA expression by binding to constitutively expressed IL-18 receptor. J Immunol. 1999; 162(3):1662-1668. (Biology). 查看参考
  5. Maruo S, Ahn HJ, Yu WG, et al. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity. J Leukoc Biol. 1997; 61(3):346-352. (Biology). 查看参考
  6. Neumann D, Martin MU. Interleukin-12 upregulates the IL-18Rbeta chain in BALB/c thymocytes. J Interferon Cytokine Res. 2001; 21(8):635-642. (Biology). 查看参考
  7. Tomura M, Maruo S, Mu J, et al. Differential capacities of CD4+, CD8+, and CD4-CD8- T cell subsets to express IL-18 receptor and produce IFN-gamma in response to IL-18. J Immunol. 1998; 160(8):3759-3765. (Biology). 查看参考
  8. Xu D, Chan WL, Leung BP, et al. Selective expression and functions of interleukin 18 receptor on T helper (Th) type 1 but not Th2 cells. J Exp Med. 1998; 188(8):1485-1492. (Biology). 查看参考
  9. Yoshimoto T, Takeda K, Tanaka T, et al. IL-12 up-regulates IL-18 receptor expression on T cells, Th1 cells, and B cells: synergism with IL-18 for IFN-gamma production. J Immunol. 1998; 161(7):3400-3407. (Biology). 查看参考
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552422 Rev. 3

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