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      Purified Mouse Anti-Rat IgG2b
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
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      BD Pharmingen™
      Rat (QC Testing)
      Mouse SJL IgG2b, κ
      Pooled rat IgG
      ELISA (Routinely Tested)
      0.5 mg/ml
      AB_395124
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      For the sandwich rat IgG2b ELISA, biotinylated mAb RG7/11.1 (Cat. No. 553898) is optimal for detection with purified anti-rat IgG2b mAb G15-337 (Cat. No. 553882) for capture. RG7/11.1 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis of intracytoplasmic IgG2b, we recommend FITC-conjugated RG7/11.1 mAb (Cat. No. 553900).

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      553897 Rev. 11
      抗体详情
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      RG7/11.1

      The RG7/11.1 antibody reacts specifically with the Fc region of rat IgG2b. It does not react with other Ig isotypes.

      This antibody is routinely tested by ELISA analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      553897 Rev. 11
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      553897 Rev.11
      报价单和参考
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      研发参考 (1)

      1. Springer TA, Bhattacharya A, Cardoza JT, Sanchez-Madrid F. Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma. 1982; 1(3):257-273. (Immunogen). 查看参考
      553897 Rev. 11

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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