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      Biotin Rat Anti-Mouse IgE
      商品详情
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      BD Pharmingen™
      Igh-7; immunoglobulin heavy chain 7 (heavy chain of IgE)
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
      Mouse IgE (pooled)
      ELISA Detection (Routinely Tested)
      0.5 mg/ml
      16020
      AB_394847
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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      推荐的实验流程

      Sandwich ELISA: Biotin Rat Anti-Mouse IgE may be used at ~ 2 µg/ml as the detection antibody coupled with Purified Rat Anti-Mouse IgE (Cat. No. 553416) as the capture antibody.  Researchers are strongly advised to titrate each reagent over a range of concentrations (e.g 1-8 µg/ml) for optimal results.  Purified Mouse IgE κ Isotype Control (Cat. No. 557079, 553481, or 557080) may be used as the ELISA standard.  Alternatively, the BD OptEIA™ Mouse IgE ELISA Set (Cat. No. 555248) is offered as a convenient sandwich ELISA product that is easy-to-use and may be used for the quantitation of soluble mouse IgE.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553414 Rev. 13
      抗体详情
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      R35-72

      The rat anti-mouse IgE antibody (clone R35-72) reacts specifically with mouse IgE of the Igh-C [a] and Igh-C [b] haplotypes. It has been reported not to react with other Ig isotypes. Detection with the rat anti-mouse IgE antibody (clone R35-72) of surface immunoglobulin on IgE-secreting hybridoma cells has also been reported.

      553414 Rev. 13
      格式详情
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      553414 Rev.13
      报价单和参考
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      研发参考 (6)

      1. Borges MS, Kumagai Y, Okumura K, Hirayama N, Ovary Z, Tada T. Allelic polymorphism of murine IgE controlled by the seventh immunoglobulin heavy chain allotype locus. Immunogenetics. 1981; 13(6):499-507. (Biology). 查看参考
      2. Driver DJ, McHeyzer-Williams LJ, Cool M, Stetson DB, McHeyzer-Williams MG. Development and maintenance of a B220- memory B cell compartment. J Immunol. 2001; 167(3):1393-1405. (Biology). 查看参考
      3. Kamijo S, Takeda H, Tokura T, et al. IL-33-Mediated Innate Response and Adaptive Immune Cells Contribute to Maximum Responses of Protease Allergen-Induced Allergic Airway Inflammation. J Immunol. 2013; 190(9):4489-4499. (Biology). 查看参考
      4. Liu FT, Albrandt K, Sutcliffe JG, Katz DH. Cloning and nucleotide sequence of mouse immunoglobulin epsilon chain cDNA. Proc Natl Acad Sci U S A. 1982; 79(24):7852-7856. (Biology). 查看参考
      5. Markowitz JS, Rogers PR, Grusby MJ, Parker DC, Glimcher LH. B lymphocyte development and activation independent of MHC class II expression.. J Immunol. 1993; 150(4):1223-33. (Biology). 查看参考
      6. Nishida Y, Kataoka T, Ishida N, et al. Cloning of mouse immunoglobulin epsilon gene and its location within the heavy chain gene cluster. Proc Natl Acad Sci U S A. 1981; 78(3):1581-1585. (Biology). 查看参考
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      553414 Rev. 13

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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