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Assay Diluent

BD OptEIA™ Assay Diluent

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The BD OptEIA™ Assay Diluent is intended for use in BD OptEIA™ ELISA Sets. The BD OptEIA Assay Diluent is a buffered protein solution containing FBS. The intended use for the BD OptEIA Assay Diluent is (a) to serve as a blocking agent for ELISA plates, (b) to serve as a diluent for ELISA standards/samples, and (c) to be used during the preparation of the Working Detector described in specific BD OptEIA ELISA Set protocols.  The BD OptEIA™ Assay Diluent should be clear, red in color and free of particulates/precipitation.

ELISA (Routinely Tested)


Store undiluted at 4°C.

Warm all reagents to room temperature (18-25°C) prior to use.Discard any remaining working solutions after use.


Please refer to the Directional Insert and the Certificate of Analysis to determine the correct coating buffer to be used and for the BD OptEIA™ ELISA specific protocol.

Warning:  Assay Diluent contains 0.003% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.


  1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  2. Please refer to for technical protocols.
555213 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.