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BD Pharmingen™ Z-IETD-FMK, Caspase-8 Inhibitor
(RUO)Flow cytometric analysis of Jurkat cells (Human T-cell leukemia; ATCC TIB-152). Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 µM Z-IETD-FMK (upper center and bottom center panels) or 20 µM of a negative control inhibitor Z-FA-FMK (upper right and bottom right panels) for 30 minutes, and then either left untreated (bottom row) or treated with 4 µM of campthothecin for 3 hours (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in camthothecin treated cells, approximately 42% of the cells were induced to undergo apoptosis and the use of the caspase-8 inhibitor Z-IETD-FMK reduced the level of apoptosis to that observed in untreated controls. Cells treated with Z-FA-FMK (Cat. No. 550411) showed similar results to the treated cells without inhibitor, indicating that the control inhibitor did not attentuate apoptosis.
BD Pharmingen™ Z-IETD-FMK, Caspase-8 Inhibitor
监管状态图例
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准备和存储
Store the lyophilized Z-IETD-FMK inhibitor at -20°C. Reconstitute the Z-IETD-FMK inhibitor in DMSO before use. The reconstituted Z-IETD-FMK inhibitor may be stored in small aliquots at -20°C.
推荐的实验流程
The Z-IETD-FMK inhibitor is designed to be used in both in vivo and in vitro cell based assays to measure the inhibition of apoptosis. Reconstitute 1.0 mg of the Z-IETD-FMK inhibitor in DMSO. A 10 mM stock solution may be made by dissolving 1.0 mg of Z-IETD-FMK in 150 µl DMSO. The final concentration of inhibitor may vary between experimental systems and investigators are encouraged to titrate the inhibitor for optimal performance. As a precautionary note, do not exceed a final DMSO concentration of 0.2% as higher levels may cause cellular toxicity and mask the effects of the caspase inhibitor.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-IETD-FMK is an irreversible and cell permeable inhibitor of caspase-8. The peptide is O-methylated in the P1 position on aspartic acid providing enhanced stability and increased cell permeability. This inhibitor can be used to inhibit caspase-8 activity and to study events downstream of caspase-8 activation. Z-IETD-FMK has a molecular weight of 654 Daltons.
研发参考 (3)
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Gregoli PA, Bondurant MC. Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors. J Cell Physiol. 1999; 178(2):133-143. (Biology). 查看参考
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Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998; 281(5381):1312-1316. (Biology). 查看参考
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Wang J, Zhen L, Klug MG, Wood D, Wu X, Mizrahi J. Involvement of caspase 3- and 8-like proteases in ceramide-induced apoptosis of cardiomyocytes. J Card Fail. 2000; 6(3):243-249. (Biology). 查看参考
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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