The HIM6 monoclonal antibody specifically binds to CD147 which is encoded by BSG. CD147 is a type I transmembrane glycoprotein (30-50 kDa) of the immunoglobulin super-gene family. Neurothelin, a blood-brain barrier-specific molecule, was clustered as CD147 in the Sixth Human Leukocyte Differentiation Antigen (HLDA) workshop. It bears homology with mouse gp42 or basigin, human "M6" or "EMMPRIN", rat OX-47 or CD-9, and avian HT7 or 5A11. CD147 is also known as Tumor cell-derived collagenase stimulatory factor (TCSF). CD147 is a molecule that is broadly expressed on cells of hematopoietic and non-hematopoietic origin. Its expression on specific cell types may be regulated by cytokines. CD147 plays a role in embryonal blood-brain barrier development and a role in integrin-mediated adhesion in brain endothelia.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.