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      Purified Rat Anti-Mouse CD51
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      Purified Rat Anti-Mouse CD51
      Expression of CD51 on mouse bone marrow myeloid cells. C57BL/6 bone marrow leukocytes were stained with either purified rat IgG1κ isotype control mAb R3-34 (Cat. No. 553922, left panel) or purified rat anti-mouse CD51 (clone RMV-7) (right panel), followed by PE-conjugated goat anti-rat Ig (Cat. No. 550767, both panels). Flow cytometry was performed on a BD FACScan™ instrument (BD Biosciences, San Jose, CA). Please note that the population of cells having the lowest SSC (erythroid and lymphoid) show little expression of CD51, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD51 positive (right panel).
      Purified Rat Anti-Mouse CD51
      Expression of CD51 on mouse bone marrow myeloid cells. C57BL/6 bone marrow leukocytes were stained with either purified rat IgG1κ isotype control mAb R3-34 (Cat. No. 553922, left panel) or purified rat anti-mouse CD51 (clone RMV-7) (right panel), followed by PE-conjugated goat anti-rat Ig (Cat. No. 550767, both panels). Flow cytometry was performed on a BD FACScan™ instrument (BD Biosciences, San Jose, CA). Please note that the population of cells having the lowest SSC (erythroid and lymphoid) show little expression of CD51, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD51 positive (right panel).
      商品详情
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      BD Pharmingen™
      Itgav; Integrin alpha-V; Integrin αV ; VNRA; Vitronectin receptor alpha
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
      Mouse (BALB/c) IL-2-activated killer (LAK) Cells
      Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)
      0.5 mg/ml
      AB_393537
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Flow cytometry:  Since this antigen is expressed at low density on the cell surface, it may be desirable to use a second-step reagent conjugated to a "bright" fluorochrome, such as PE goat anti-rat Ig (Cat. No. 550767).

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      550024 Rev. 7
      抗体详情
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      RMV-7

      The RMV-7 monoclonal antibody specifically binds to CD51, the 140 kDa integrin αV chain. Heterodimers of CD51 with several integrin β chains function as receptors for extracellular matrix proteins. CD51/CD61 (αVβ3 integrin, vitronectin receptor) mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, von Willebrand factor, and CD31 (PECAM-1). CD51 is expressed on activated T lymphocytes, polymorphonuclear granulocytes, blastocysts, and osteoclasts. CD51 is reportedly not detectable on mouse platelets using either H9.2B8 or RMV-7 antibody clones. CD51 also forms heterodimers with CD29 (integrin β1), integrins β5, β6, and β8 chains. αV integrins have diverse functions in development and homeostasis. The RMV-7 antibody reportedly blocks LAK-cell binding to vitronectin, fibronectin, fibrinogen, and CD31. Furthermore, the RMV-7 antibody reportedly inhibits LAK-cell cytoxicity against certain target cells by interfering with the binding of LAK cells to their target cells.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      550024 Rev. 7
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550024 Rev.7
      报价单和参考
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      研发参考 (8)

      1. Bader BL, Rayburn H, Crowley D, Hynes RO. Extensive vasculogenesis, angiogenesis, and organogenesis precede lethality in mice lacking all alpha v integrins. Cell. 1998; 95(4):507-519. (Biology). 查看参考
      2. Frieser M, Hallmann R, Johansson S, Vestweber D, Goodman SL, Sorokin L. Mouse polymorphonuclear granulocyte binding to extracellular matrix molecules involves beta 1 integrins. Eur J Immunol. 1996; 26(12):3127-3136. (Biology). 查看参考
      3. Moulder K, Roberts K, Shevach EM, Coligan JE. The mouse vitronectin receptor is a T cell activation antigen. J Exp Med. 1991; 173(2):343-347. (Biology). 查看参考
      4. Nakamura I, Pilkington MF, Lakkakorpi PT, et al. Role of alpha(v)beta(3) integrin in osteoclast migration and formation of the sealing zone. J Cell Sci. 1999; 112(22):3985-3993. (Biology). 查看参考
      5. Narumiya S, Abe Y, Kita Y, et al. Pre-B cells adhere to fibronectin via interactions of integrin alpha 5/alpha V with RGDS as well as of integrin alpha 4 with two distinct V region sequences at its different binding sites. Int Immunol. 1994; 6(1):139-147. (Biology: Blocking). 查看参考
      6. Piali L, Hammel P, Uherek C, et al. CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium. J Cell Biol. 1995; 130(2):451-460. (Biology: Blocking). 查看参考
      7. Schultz JF, Armant DR. Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor. J Biol Chem. 1995; 270(19):11522-11531. (Biology). 查看参考
      8. Takahashi K, Nakamura T, Koyanagi M, et al. A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction. J Immunol. 1990; 145(12):4371-4379. (Immunogen: Blocking, Immunoprecipitation). 查看参考
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      550024 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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