Flow cytometric analysis of CD104 expression on A431 cells, a human epidermal carcinoma cell line. A431 cells were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 555846; dashed line histogram) or Purified Rat Anti-Human CD104 (Cat. No. 555719; solid line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable A431 cells. Flow cytometry was performed on a BD FACScan™ system.
Flow cytometric analysis of CD104 expression on A431 cells, a human epidermal carcinoma cell line. A431 cells were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 555846; dashed line histogram) or Purified Rat Anti-Human CD104 (Cat. No. 555719; solid line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable A431 cells. Flow cytometry was performed on a BD FACScan™ system.
商品详情
BD Pharmingen™
Integrin β4 chain; Integrin beta 4; ITGB4; GP150; TSP-180
Human (QC Testing)
Rat F344, also known as Fischer, CDF IgG2b, κ
Human CD104 Protein
Flow cytometry (Routinely Tested)
0.5 mg/ml
V BP480, S247
3691
AB_396062
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
准备和存储
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
Stain Buffer (FBS) RUO
尺寸
500 mL
品类编号
554656
Stain Buffer (BSA) RUO
尺寸
500 mL
品类编号
554657
Purified Rat IgG2b, κ Isotype Control RUO
尺寸
0.1 mg
品类编号
555846
FITC Goat Anti-Mouse IgG/IgM RUO
尺寸
0.5 mg
品类编号
555988
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555719 Rev. 9
抗体详情
439-9B
The 439-9B monoclonal antibody specifically recognizes CD104, integrin β4 chain, a 205 kDa transmembrane glycoprotein, which associates with CD49f (integrin α6 chain) to form the α6/β4 (CD49f/CD104) complex. It is expressed on epithelial cells, Schwann cells, and some tumor cells. The CD49f/CD104 complex is located in the hemidesmosomes of the epidermis, suggesting its role in epidermal cell-basement membrane adhesion. The clone 439-9B was clustered as CD104 at the fifth Human Leucocyte Differentiation Antigen International Workshop. It may be used for immunoprecipitation, immunoblotting and immunohistochemistry on frozen tissue sections.
555719 Rev. 9
格式详情
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555719 Rev.9
报价单和参考
研发参考 (7)
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Falcioni R, Sacchi A, Resau J, Kennel SJ. Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue.. Cancer Res. 1988; 48(4):816-21. (Immunogen). 查看参考
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Hemler ME, Crouse C, Sonnenberg A. Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines. J Biol Chem. 1989; 264(11):6529-6535. (Biology). 查看参考
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Jones JC, Kurpakus MA, Cooper HM, Quaranta V. A function for the integrin alpha 6 beta 4 in the hemidesmosome. Cell Regul. 1991; 2(6):427-438. (Biology). 查看参考
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Mariani Costantini R, Falcioni R, Battista P, et al. Integrin (alpha 6/beta 4) expression in human lung cancer as monitored by specific monoclonal antibodies.. Cancer Res. 1990; 50(18):6107-12. (Biology). 查看参考
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Sonnenberg A, Calafat J, Janssen H, et al. Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion.. J Cell Biol. 1991; 113(4):907-17. (Biology). 查看参考
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Tamura RN, Rozzo C, Starr L, et al. Epithelial integrin alpha 6 beta 4: complete primary structure of alpha 6 and variant forms of beta 4.. J Cell Biol. 1990; 111(4):1593-604. (Biology). 查看参考
555719 Rev. 9
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
