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      Purified Rat Anti-Human and Viral IL-10
      商品详情
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      BD Pharmingen™
      CSIF; Cytokine synthesis inhibitory factor; TGIF
      Human (QC Testing), Viral (Tested in Development)
      Rat IgG1
      Recombinant Human IL-10
      ELISA Capture (Routinely Tested), Intracellular block/flow cytometry (Tested During Development), Western blot (Reported)
      0.5 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      Blocking Control for Intracellular Staining: The purified JES3-9D7 antibody can be used as a blocking control to demonstrate specificity of

      IL-10 staining by PE-conjugated JES3-9D7 antibody (Cat. No. 554498). To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1-10 µg of unlabeled JES3-9D7 antibody (Cat. No. 554496) for 20 minutes at 4°C, prior to staining with PE-JES3-9D7 antibody (e.g., 0.1 - 0.5 µg mAb/1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

      Western Blot: The JES3-9D7 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554497 Rev. 3
      抗体详情
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      JES3-9D7

      The JES3-9D7 monoclonal antibody specifically reacts with human IL-10 (Interleukin-10) and viral IL-10. The immunogen used to generate the JES3-9D7 hybridoma was recombinant human IL-10 expressed by COS cells. IL-10 is also known as CSIF (Cytokine synthesis inhibitory factor) and (TGIF) T-cell growth inhibitory factor. IL-10 is expressed by various cell types including activated monocytes, macrophages, dendritic cells, mast cells, granulocytes, and lymphocytes. IL-10 is a pleiotropic cytokine that can downregulate proinflammatory immune responses, such as Th1-like responses, while promoting other responses including B cell proliferation and antibody production.

      554497 Rev. 3
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554497 Rev.3
      报价单和参考
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      View product citations for antibody "554497" on CiteAb

      研发参考 (5)

      1. Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Biology: ELISA). 查看参考
      2. Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Biology: ELISA). 查看参考
      3. Burdin N, Peronne C, Banchereau J, Rousset F. Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10. J Exp Med. 1993; 177(2):295-304. (Biology: ELISA). 查看参考
      4. Gotlieb WH, Abrams JS, Watson JM, Velu TJ, Berek JS, Martinez-Maza O. Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers. Cytokine. 1992; 4(5):385-390. (Biology: ELISA). 查看参考
      5. Yssel H, De Waal Malefyt R, Roncarolo MG, et al. IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells. J Immunol. 1992; 149(7):2378-2384. (Biology: ELISA). 查看参考
      查看所有文件 (5) 查看更少内容
      554497 Rev. 3

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.