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Functional studies of TC11-18H10 mAb by neutralization of mouse IL-17A bioactivity. Recombinant mouse IL-17A (2 ng/well) was pre-incubated with various amounts of TC11-18H10 mAb or medium as a control in a 96-well plate for 1 hour at 37°C. Following the incubation, NIH-3T3 cells were added at 5x10^4 cells per well and incubated at 37°C. After 24 hours of incubation, the supernatant was harvested and the concentration of IL-6 was quantified using BD™ Cytometric Bead Array Mouse IL-6 Flex Set (Cat. No. 558301).
BD Pharmingen™ Purified NA/LE Rat Anti-Mouse IL-17A
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准备和存储
推荐的实验流程
ELISA: Purified TC11-18H10 antibody (Cat. No. 560268) can be useful as a capture antibody for sandwich ELISA measuring mouse IL-17A protein levels. Purified TC11-18H10 antibody can be paired with biotinylated TC11-8H4 (Cat. No. 555067) as the detector antibody, with recombinant mouse IL-17A protein as the standard. Investigators are encouraged to titrate purified TC11-18H10 from 2.0 µg/mL - 0.5 µg/mL for optimal concentration determination for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant mouse IL-17A are suggested from 2,000 - 15 pg/mL.
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The TC11-18H10 monoclonal antibody specifically binds to recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses. Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro; it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity. Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRαβ+CD4-CD8- thymocytes.
研发参考 (4)
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Kennedy J, Rossi DL, Zurawski SM, et al. Mouse IL-17: a cytokine preferentially expressed by alpha beta TCR + CD4-CD8-T cells. J Interferon Cytokine Res. 1996; 16(8):611-617. (Biology). 查看参考
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 查看参考
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Schwarzenberger P, La Russa V, Miller A, et al. IL-17 stimulates granulopoiesis in mice: use of an alternate, novel gene therapy-derived method for in vivo evaluation of cytokines. J Immunol. 1998; 161(11):6383-6389. (Biology). 查看参考
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Yen D, Cheung J, Scheerens H et al. IL-23 is essential for T cell–mediated colitis and promotes inflammation via IL-17 and IL-6. J Clin Invest. 2006; 116(5):1310-1316. (Methodology: Neutralization). 查看参考
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