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Reagents
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蛋白质印迹试剂
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Single-Cell Multiomics Reagents
- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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Functional Assays
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显微成像试剂
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Cell Preparation and Separation Reagents
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- BD® AbSeq Assay
- BD Rhapsody™ 附件试剂盒
- BD® Single-Cell Multiplexing Kit
- BD Rhapsody™ Targeted mRNA Kits
- BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit
- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The JJ319 monoclonal antibody specifically recognizes CD28, which is expressed on virtually all T lymphocytes bearing αβ T-cell receptors (TCR), on most γδ TCR-bearing T cells, and on a subset of NK cells. In the thymus, CD28 expression is developmentally regulated during the maturation of αβ TCR-bearing T cells. CD28 is a costimulatory receptor required for activation of T cells; its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble JJ319 mAb costimulates the proliferative responses and IL-2 production of CD4+ and CD8+ T cells activated by anti-αβ-TCR mAb R73 (Cat. no. 554910). The alternate anti-rat CD28 mAb JJ316 (Cat. No. 554992) is capable of directly stimulating T cells in vitro and in vivo.
研发参考 (4)
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Bluestone JA. New perspectives of CD28-B7-mediated T cell costimulation. Immunity. 1995; 2(6):555-559. (Biology). 查看参考
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Mitnacht R, Tacke M, Hunig T. Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. Eur J Immunol. 1995; 25(2):328-332. (Biology). 查看参考
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Tacke M, Clark GJ, Dallman MJ, Hunig T. Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ester. J Immunol. 1995; 154(10):5121-5127. (Immunogen: (Co)-stimulation, Immunoprecipitation). 查看参考
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Tacke M, Hanke G, Hanke T, Hunig T. CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur J Immunol. 1997; 27(1):239-247. (Clone-specific: (Co)-stimulation, Immunoprecipitation). 查看参考
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