准备和存储
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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配套商品
The JJ316 antibody reacts with CD28 on virtually all T lymphocytes bearing αβT-cell receptors (TCR), on most γδTCR-bearing T cells, and on a subset of NK cells. In the thymus, CD28 expression is developmentally regulated during the maturation of αβTCR-bearing T cells. CD28 is a costimulatory receptor required for activation of T cells; its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble JJ316 enhances the proliferative responses and IL-2 production by T cells activated by anti-aß-TCR mAb R73 (Cat. no. 554910). Furthermore, at high concentrations, cross-linked JJ316 mAb stimulates resting CD4+ and CD8+T cells to proliferate, secrete IL-2, express activation markers, and gain CTL activity in the absence of signaling through the TCR. In vivo administration of mAb JJ316 results in blastogenic transformation of peripheral CD4+ cells and dramatic lymphocytosis. The alternate anti-rat CD28 mAb JJ319 (Cat. no. 554993) cannot directly activate T cells, although it has in vitro costimulatory activity.
研发参考 (4)
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Bluestone JA. New perspectives of CD28-B7-mediated T cell costimulation. Immunity. 1995; 2(6):555-559. (Biology). 查看参考
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Mitnacht R, Tacke M, Hunig T. Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. Eur J Immunol. 1995; 25(2):328-332. (Biology). 查看参考
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Tacke M, Clark GJ, Dallman MJ, Hunig T. Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ester. J Immunol. 1995; 154(10):5121-5127. (Immunogen). 查看参考
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Tacke M, Hanke G, Hanke T, Hunig T. CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur J Immunol. 1997; 27(1):239-247. (Clone-specific: (Co)-stimulation, Stimulation). 查看参考
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