Purified NA/LE Mouse Anti-Human CD44

This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD44

Name: Purified NA/LE Mouse Anti-Human CD44
Brand: BD Pharmingen™
SKU: 550990

克隆 515

(RUO)

Flow cytometric analysis of CD44 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified NA/LE Mouse Anti-Human CD44 antibody (Cat. No. 550990; solid line histogram) or Purified NA/LE Mouse IgG1 κ Isotype Control (Cat. No. 554721; dashed line histogram). Secondary staining was carried out with FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) and erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™.

光谱浏览器

协议库

科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: Pgp-1; CSPG8; ECMR-III; Epican; H-CAM; HCELL; Hermes; HUTCH-1

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human EBV-transformed Arent B Lymphoblastoid Cell Line

Application: Flow cytometry (Routinely Tested)

Concentration: 1.0 mg/ml

Entrez Gene ID: 960

RRID: AB_394001

Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.

Regulatory Status: RUO

准备和存储

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

配套商品

Stain Buffer (FBS) RUO

尺寸 500 mL 品类编号 554656

Stain Buffer (BSA) RUO

尺寸 500 mL 品类编号 554657

Lysing Solution 10X Concentrate CFDA_IVD

尺寸 100 mL 品类编号 349202

Lysing Buffer RUO

尺寸 100 mL 品类编号 555899

Purified NA/LE Mouse IgG1 κ Isotype Control RUO

尺寸 0.5 mg 品类编号 554721

FITC Goat Anti-Mouse IgG/IgM RUO

尺寸 0.5 mg 品类编号 555988

查看详情

550990 Rev. 6

抗体详情

515

The 515 monoclonal antibody specifically binds to CD44. CD44 is an 80-95 kDa type I transmembrane glycoprotein, also known as Phagocytic glycoprotein-1 (Pgp-1), or Extracellular matrix receptor type III (ECMR-III). CD44 is a member of the hyaladherin family of hyaluronan-binding proteins, with structural similarities to selectins. CD44 is the receptor for hyaluronic acid. CD44 is expressed on leucocytes, erythrocytes, epithelial cells and weakly on platelets. CD44 has functional roles in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation. The 515 monoclonal antibody can reportedly block cellular adhesion to hyaluronic acid.

550990 Rev. 6

格式详情

NA/LE

NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

格式: NA/LE

550990 Rev.6

报价单和参考

View product citations for antibody "550990" on CiteAb

研发参考 (5)

Kansas GS, Muirhead MJ, Dailey MO. Expression of the CD11/CD18, leukocyte adhesion molecule 1, and CD44 adhesion molecules during normal myeloid and erythroid differentiation in humans. Blood. 1990; 76(12):2483-2492. (Biology). 查看参考
Kansas GS, Tedder TF. Transmembrane signals generated through MHC class II, CD19, CD20, CD39, and CD40 antigens induce LFA-1-dependent and independent adhesion in human B cells through a tyrosine kinase-dependent pathway. J Immunol. 1991; 147(12):4094-4102. (Biology). 查看参考
Kansas GS, Wood GS, Dailey MO. A family of cell-surface glycoproteins defined by a putative anti-endothelial cell receptor antibody in man.. J Immunol. 1989; 142(9):3050-7. (Clone-specific). 查看参考
Oostendorp RAJ, Spitzer E, Reisbach G, Dörmer P. CD44 Workshop: CD44 modulates adhesive behavior of hematopoietic progenitor cells. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:378-379.
Patel DD, Liao HX, Haynes BF. CD44 workshop panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:373-375.

查看所有文件 (5)

550990 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.