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Purified Mouse Anti-Rat IgM
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BD Pharmingen™
Rat (QC Testing)
Mouse IgG1, κ
Pooled Rat Ig
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.


For the sandwich rat IgM ELISA, purified G53-238 mAb may be used for capture with biotin-conjugated G53-238 mAb (Cat. No. 553886) for detection. For flow cytometric detection of intracytoplasmic IgM, we recommend FITC-conjugated G53-238 mAb (Cat. No. 553887).


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553885 Rev. 13
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The G53-238 antibody reacts specifically with rat IgM monomers and pentamers. It does not react with other Ig isotypes. G53-238 antibody has not been shown to stimulate B-cell proliferation.

553885 Rev. 13
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
553885 Rev.13

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.