Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
China (Chinese)
Profile Icon Profile Icon
登录/注册 UserName
产品

解决方案

发现&学习

资源&工具

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-Rat CD90/Mouse CD90.1
      Purified Mouse Anti-Rat CD90/Mouse CD90.1
      Two color analysis of the expression of CD90 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with PE mouse anti-rat CD3 (clone G4.18) (Cat. No. 554833) and purified mouse anti-rat CD90/mouse CD90.1 (clone OX-7) (right panel), followed by FITC rat anti-mouse IgG1 (clone A85-1) (Cat. No. 553443). Flow cytometry was performed on a BD FACScan™ instrument.
      Purified Mouse Anti-Rat CD90/Mouse CD90.1
      Two color analysis of the expression of CD90 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with PE mouse anti-rat CD3 (clone G4.18) (Cat. No. 554833) and purified mouse anti-rat CD90/mouse CD90.1 (clone OX-7) (right panel), followed by FITC rat anti-mouse IgG1 (clone A85-1) (Cat. No. 553443). Flow cytometry was performed on a BD FACScan™ instrument.
      商品详情
      Down Arrow Up Arrow


      BD Pharmingen™
      Rat Thy-1; Mouse Thy-1.1
      Rat (QC Testing), Mouse (Tested in Development), Rabbit,Guinea Pig (Reported)
      Mouse BALB/c IgG1, κ
      Rat Thymocyte Thy-1 Antigen
      Flow cytometry (Routinely Tested), Immunohistochemistry-zinc-fixed (Tested During Development), Electron microscopy, Immunohistochemistry-frozen, Immunoprecipitation, Western blot (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
      0.5 mg/ml
      AB_395586
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      显示较多信息 blue down arrow 显示较少信息 blue up arrow
      554895 Rev. 13
      抗体详情
      Down Arrow Up Arrow
      OX-7

      CD90 (Thy-1) is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction.  The OX-7 monoclonal antibody specifically binds to rat CD90 reported to be expressed by hematopoietic stem cells, early myeloid and erythroid cells, immature B lymphocytes in the bone marrow and peripheral lymphoid organs, thymocytes, recent thymic emigrants (a subset of CD45RC- peripheral T lymphocytes), neurons, glomerular mesangial cells, endothelium at inflammatory sites, mast cells, and dendritic cells. Rat dendritic epidermal T cells (DEC) have been reported to be CD90 (Thy-1) negative, unlike those of the mouse.

      The OX-7 clone has been reported to crossreact with the mouse CD90.1 (Thy-1.1) alloantigen of the AKR/J and PL strains, but not CD90.2 (Thy-1.2) found on many mouse strains. In the mouse, CD90 is found on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), hematopoietic stem cells, and neurons, but not B lymphocytes. In addition, there is evidence that CD90 mediates adhesion of mouse thymocytes to mouse thymic stroma. The OX-7 clone has also been reported to crossreact with rabbit and guinea pig thymus, brain, and intestine.

      554895 Rev. 13
      格式详情
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554895 Rev.13
      报价单和参考
      Down Arrow Up Arrow

      研发参考 (21)

      1. Bañuls MP, Alvarez A, Ferrero I, Zapata A, Ardavin C. Cell-surface marker analysis of rat thymic dendritic cells. Immunology. 1993; 79(2):298-304. (Biology). 查看参考
      2. Campbell DG, Gagnon J, Reid KB, Williams AF. Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region. Biochem J. 1981; 195(1):15-30. (Biology). 查看参考
      3. Chen-Woan M, Delaney CP, Fournier V, et al. In vitro characterization of rat bone marrow-derived dendritic cells and their precursors. J Leukoc Biol. 1996; 59(2):196-207. (Biology). 查看参考
      4. Crook K and Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. 1996; 4:235-246. (Biology). 查看参考
      5. Dráberová L, Amoui M, and Dráber P. Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains. Immunology. 1996; 87(1):141-148. (Clone-specific: Activation, Western blot). 查看参考
      6. Eitner F, Westerhuis R, Burg M, et al. Role of interleukin-6 in mediating mesangial cell proliferation and matrix production in vivo. Kidney Int. 1997; 51(1):69-78. (Biology). 查看参考
      7. Elbe A, Kilgus O, Hünig T, and Stingl G. T-cell receptor diversity in dendritic epidermal T cells in the rat. J Invest Dermatol. 1993; 102:74-79. (Biology). 查看参考
      8. Ferrero I, Bañnuls M, Alvarez A, Ardavín C. Rat thymic dendritic cells: cell surface marker variations in culture. Immunol Lett. 1993; 37(2-3):241-247. (Biology). 查看参考
      9. Garnett D, Barclay AN, Carmo AM, Beyers AD. The association of the protein tyrosine kinases p56lck and p60fyn with the glycosyl phosphatidylinositol-anchored proteins Thy-1 and CD48 in rat thymocytes is dependent on the state of cellular activation. Eur J Immunol. 1993; 23(10):2540-2544. (Clone-specific: Immunoprecipitation). 查看参考
      10. Hasslen SR, Burns AR, Simon SI, et al. Preservation of spatial organization and antigenicity of leukocyte surface molecules by aldehyde fixation: flow cytometry and high-resolution FESEM studies of CD62L, CD11b, and Thy-1. J Histochem Cytochem. 1996; 44(10):1115-1122. (Clone-specific: Electron microscopy). 查看参考
      11. He HT, Naquet P, Caillol D, Pierres M. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism. J Exp Med. 1991; 173(2):515-518. (Biology). 查看参考
      12. Hermans MH, Opstelten D. In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J Histochem Cytochem. 1991; 39(12):1627-1634. (Clone-specific: Immunohistochemistry). 查看参考
      13. Hosseinzadeh H, Goldschneider I. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J Immunol. 1993; 150(5):1670-1679. (Biology). 查看参考
      14. Ishizu A, Ishikura H, Nakamaru Y et al. Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int Immunol. 1995; 7:1939-1947. (Clone-specific: Immunoprecipitation). 查看参考
      15. Kawachi H, Orikasa M, Matsui K, et al. Epitope-specific induction of mesangial lesions with proteinuria by a MoAb against mesangial cell surface antigen. Clin Exp Immunol. 1992; 88(3):399-404. (Clone-specific: Western blot). 查看参考
      16. Liu L, Zhang M, Jenkins C, MacPherson GG. Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression. J Immunol. 1998; 161(3):1146-1155. (Biology). 查看参考
      17. Mason DW, Williams AF. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J. 1980; 187(1):1-20. (Immunogen). 查看参考
      18. Morita H, Isobe K, Cai Z, et al. Thy-1 antigen mediates apoptosis of rat glomerular cells in vitro and in vivo. Nephron. 1996; 73(2):293-298. (Biology: Apoptosis). 查看参考
      19. Nakashima I, Zhang YH, Rahman SM, et al. Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death. J Immunol. 1991; 147(4):1153-1162. (Biology: Activation). 查看参考
      20. Paul LC, Rennke HG, Milford EL, and Carpenter CB. Thy-1.1 in glomeruli of rat kidneys. Kidney Int. 1984; 25:771-777. (Clone-specific: Electron microscopy, Immunohistochemistry). 查看参考
      21. Payer E, Elbe A, Stingl G. Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells. J Immunol. 1991; 146(8):2536-2543. (Biology). 查看参考
      查看所有文件 (21) 查看更少内容
      554895 Rev. 13

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback