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Two-color analysis of CD24 expression on B-lineage cells in rat bone marrow. Cells flushed from Lewis femurs were stained with PE-conjugated anti-rat CD45R B-lymphocyte-specific mAb HIS24 (Cat. no. 554881) and either purified isotype control mAb G155-228 (Cat. no. 553472, left panel) or purified mAb HIS50 (right panel), followed by FITC-conjugated anti-mouse IgM mAb II/41 (Cat. no. 553437). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Rat CD24
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The HIS50 monoclonal antibody specifically binds to CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein. In the rat, Cd24 mRNA is found in a variety of epithelia, neural tissue, lymph node germinal centers and thymus. The HIS50 antibody detects CD24 on most B-lineage cells in the bone marrow, on most highly surface IgM (sIgM)-positive B lymphocytes in the blood and spleen, and on marginal-zone B cells in the spleen and lymph node. Variable levels of CD24 are detected on B cells that expresses low levels of sIgM and on follicular B lymphocytes. The HIS50 antibody does not detect CD24 on rat thymocytes nor peripheral T lymphocytes, NK cells, or granulocytes. The HIS50 antibody crossreacts with intracellular antigen(s) in human lymphocytes.
研发参考 (3)
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Akashi T, Shirasawa T, Hirokawa K.. Gene expression of CD24 core polypeptide molecule in normal rat tissues and human tumor cell lines.. Virchows Arch. 1994; 425(4):399-406. (Biology: Bioassay). 查看参考
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Hermans MH, Deenen GJ, De Boer N, Bo W, Kroese FG, Opstelten D. Expression of HIS50 Ag: a rat homologue of mouse heat-stable antigen and human CD24 on B lymphoid cells in the rat. Immunology. 1997; 90(1):14-22. (Immunogen: Immunofluorescence, Immunohistochemistry). 查看参考
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Pearce EI, Ammerlaan WA, Opstelten D.. Characterization of rat HSA/CD24 protein bearing the B lineage-restricted epitope recognized by MAb HIS50.. Hybridoma. 1996 February; 15(1):33-42. (Clone-specific: Western blot). 查看参考
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