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- BD Rhapsody™ TCR/BCR Profiling Assays
- BD® OMICS-Guard Sample Preservation Buffer
- BD Rhapsody™ ATAC-Seq Assays
- BD Rhapsody™ TCR/BCR Next Multiomic Assays
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BD Transduction Laboratories™ Purified Mouse Anti-p120 Catenin (pY228)
克隆 21a/p120 Catenin (pY228)
(RUO)Human Endothelial cells were treated with#1 mM pervanadate for 15 min at 37°C. The top panel was probed with p120 Catenin (cat.#610133) and the bottom panel was probed with p120 Catenin (pY228) (cat.#612536).
Eahy cells were serum starved and treated with pervanadate (1mM) for 20 min., then fixed in 3.75% paraformaldehyde with 0.2% Triton X-100. Immunofluorescent staining was performed with p120 Catenin (cat.#610133) and p120 Catenin (pY228) HeLa (cat.#612536).
BD Transduction Laboratories™ Purified Mouse Anti-p120 Catenin (pY228)
BD Transduction Laboratories™ Purified Mouse Anti-p120 Catenin (pY228)
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The membrane associated protein pp120 Src substrate (p120 Catenin, p120cas)was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation. It shares structural similarity with the Drosophila Armadillo protein and the vertebrate β-catenin and γ-catenin proteins. This similarity is evidenced by its characteristic Arm domain that is composed of 42-amino acid motif repeats. In the cell, p120 Catenin is localized to the E-Cadherin/catenins cell adhesion complex. Like β- and γ-catenin, p120 Catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain and may similarly interact with other Cadherins. It exists as four isoforms that range in size from 90-115kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions. Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 Catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation. Thus, p120 Catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signaling molecules, such as PTP1C.
研发参考 (2)
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Mariner DJ, Anastasiadis P, Keilhack H, Bohmer FD, Wang J, Reynolds AB. Identification of Src phosphorylation sites in the catenin p120ctn. J Biol Chem. 2001; 276(30):28006-28013. (Biology). 查看参考
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Reynolds AB, Daniel J, McCrea PD, Wheelock MJ, Wu J, Zhang Z. Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes. Mol Cell Biol. 1994; 14(12):8333-8342. (Biology). 查看参考
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