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The 2-69 monoclonal antibody specifically binds to 2B4 which is also known as CD244. CD244 is an approximately 63-70 kDa type I transmembrane glycoprotein. It is a SLAM submember (SLAMF4) and CD2 family member of the immunoglobulin superfamily. CD244 was originally identified in the mouse as a non-MHC-restricted cytotoxicity mediator present on NK cells and CD8+ T cells. In humans, CD244 is expressed on NK cells, γδ T cells, subsets of effector and memory CD8+ T cells, monocytes, eosinophils and basophils. This expression pattern suggests a broad role for CD244 in the regulation of leukocyte activation. CD244 binds CD48 with high affinity. CD244 may serve both stimulatory and inhibitory functions depending on a number of factors. These include the intensity and duration of CD244 interactions with its ligands as well as the presence of other signals generated through different receptor-ligand interactions. 2B4 was clustered as CD244 in the VIIth HLDA workshop.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (6)
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Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
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Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002.
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Mathew PA, Garni-Wagner BA, Land K, et al. Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells. J Immunol. 1993; 151(10):5328-5337. (Biology). 查看参考
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Nakajima H, Cella M, Langen H, Friedlein A, Colonna M. Activating interactions in human NK cell recognition: the role of 2B4-CD48. Eur J Immunol. 1999; 29(5):1676-1683. (Biology). 查看参考
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Sivori S, Parolini S, Falco M, et al. 2B4 functions as a co-receptor in human NK cell activation. Eur J Immunol. 2000; 30(3):787-793. (Biology). 查看参考
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Tangye SG, Lazetic S, Woollatt E, Sutherland GR, Lanier LL, Phillips JH. Cutting edge: human 2B4, an activating NK cell receptor, recruits the protein tyrosine phosphatase SHP-2 and the adaptor signaling protein SAP. J Immunol. 1999; 162(12):6981-6985. (Biology). 查看参考
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