Purified Mouse Anti-Human CD177

BD Pharmingen™ Purified Mouse Anti-Human CD177

Name: Purified Mouse Anti-Human CD177
Brand: Purified Mouse Anti-Human CD177
SKU: 551899

克隆 MEM-166 (RUO)

Profile of peripheral blood granulocytes analyzed by flow cytometry. Second step staining with Cat. No. 555988.

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实验方案

科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: Human neutrophil alloantigen 2a; HNA-2a; NB1; PRV-1; PRV1

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Human Granulocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

Workshop Number: VI M-M17, BP-609; VII 70510

RRID: AB_394289

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

商品通知

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

配套商品

Purified Mouse IgG1, κ Isotype Control RUO

尺寸 0.1 mg 品类编号 555746

FITC Goat Anti-Mouse IgG/IgM RUO

尺寸 0.5 mg 品类编号 555988

551899 Rev. 3

抗体详情

MEM-166

The MEM-166 monoclonal antibody specifically binds to CD177 which is also known as NB1 (Neutrophil-specific antigen), HNA-2a (Human neutrophil alloantigen 2a), and PRV1 (Polycythemia rubra vera 1). CD177 is a glycophosphatidylinositol (GPI)-anchored plasma membrane glycoprotein (56-64 kDa). CD177 is expressed on subpopulations of neutrophils, neutrophillic metamyelocytes and myelocytes from 89 to 97% of healthy individuals. It is also expressed on secondary granules. Its expression is upregulated on granulocytes stimulated with the chemotactic peptide f-met-leu-phe (fMLP). Although the function of CD177 has not been fully established, some reports suggest its possible role as receptor molecule involved in leucocyte migration.

551899 Rev. 3

格式详情

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

格式: Purified

551899 Rev.3

报价单和参考

View product citations for antibody "551899" on CiteAb

研发参考 (4)

Clement LT, Lehmeyer JE, Gartland GL.. Identification of neutrophil subpopulations with monoclonal antibodies. Blood. 1983 February; 61(2):326-332. (Biology). 查看参考
Matsuo K, Lin A, Procter JL, Clement L, Stroncek D. Variations in the expression of granulocyte antigen NB1. Transfusion. 2000 June; 40(6):654-662. (Biology). 查看参考
Stroncek DF, Shankar R, Litz C, Clement L. The expression of the NB1 antigen on myeloid precursors and neutrophils from children and umbilical cords. Transfus Med. 1998 June; 8(2):119-123. (Biology). 查看参考
Stroncek DF, Shankar RA, Noren PA, Herr GP, Clement LT. Analysis of the expression of NB1 antigen using two monoclonal antibodies. Transfusion. 1996 February; 36(2):168-174. (Biology). 查看参考

查看所有文件 (4)

551899 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.