Old Browser
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Western blot analysis of human CD110 (Thrombopoietin receptor) expressed by human HEL92.1.7 cells and platelets. Lysates prepared from human HEL92.1.7 cells and platelets were SDS-PAGE electrophoresed and transferred to membranes. They were then probed using Purified Mouse Anti-Human CD110 antibody (Cat. No. 562137) at concentrations of 0.25 (lane 1), 0.125 (lane 2) and 0.06 (lane 3) μg/ml. CD110 is identified as approximately 65-72 kDa bands from HEL cells (Left Panel) and 80-90 kDa bands from platelets (Right Panel). The molecular masses observed for the Thrombopoietin Receptor protein using the 1.78.1 antibody for Western blotting may vary due to splice variations and/or different post-translational modifications of the receptor obtained from different cell types.
BD Pharmingen™ Purified Mouse anti-Human CD110
监管状态图例
未经BD明确书面授权,严禁使用未经许可的任何商品。
准备和存储
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The 1.78.1 monoclonal antibody specifically binds to the human Thrombopoietin Receptor (TPO-R) that is also know as the Myeloproliferative leukemia protein (c-Mpl) or CD110. CD110 is a type I transmembrane protein and a member of the hematopoietin receptor family. It is expressed on hematopoietic stem cells, a subfraction of hematopoietic precursor cells, cells of the megakaryocytic lineage and platelets. CD110 serves as a receptor for thrombopoietin. Upon binding of thrombopoietin to CD110, megakaryocyte proliferation and differentiation is induced and stem cells are protected from apoptosis.
研发参考 (4)
-
Abbott C, Huang G, Ellison AR, et al. Mouse monoclonal antibodies against human c-Mpl and characterization for flow cytometry applications.. Hybridoma (Larchmt). 2010; 29(2):103-13. (Clone-specific: Flow cytometry). 查看参考
-
Broudy VC, Lin NL, Fox N, Taga T, Saito M, Kaushansky K. Thrombopoietin stimulates colony-forming unit-megakaryocyte proliferation and megakaryocyte maturation independently of cytokines that signal through the gp130 receptor subunit. Blood. 1996; 88(6):2026-2032. (Biology). 查看参考
-
Deng B, Banu N, Malloy B, et al. An agonist murine monoclonal antibody to the human c-Mpl receptor stimulates megakaryocytopoiesis. Blood. 1998; 92(6):1981-1988. (Biology). 查看参考
-
Gotoh A, Ritchie A, Takahira H, Broxmeyer HE. Thrombopoietin and erythropoietin activate inside-out signaling of integrin and enhance adhesion to immobilized fibronectin in human growth-factor-dependent hematopoietic cells. Ann Hematol. 1997; 75(5-6):207-213. (Biology). 查看参考
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.