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Expression of CD209 on peripheral blood monocyte-derived dendritic cells. Adherent peripheral blood mononuclear cells were cultured for 7 days with the recombinant human cytokines, GM-CSF (Cat. No. 550068) and IL-4 (Cat. No. 554605). Then the cultured dendritic cells were stained with either PerCP-Cy™5.5 Mouse IgG2b, κ Isotype Control (Cat. No. 558304, thin histogram) or PerCP-Cy™5.5 Mouse Anti-Human CD209 (bold histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of vialbe cells. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ PerCP-Cy™5.5 Mouse Anti-Human CD209
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商品通知
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
- PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
配套商品
The DCN46 antibody specifically binds to dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN or CD209), a type-II membrane protein of approximately 44 kDa with a mannose-binding C-type lectin domain. It is highly expressed on dendritic cells in mucosal tissues. Its sequence is identical to the HIV-1 envelope gp120-binding C-type lectin, and reports suggest that DC-SIGN binds to HIV-1 gp120 and effectively transmits infectious HIV-1 to resting T lymphocytes expressing CD4 and chemokine receptors. The C-type lectin domain of DC-SIGN is also capable of binding other pathogenic viruses, bacteria, and parasites. Reports also suggest that DC-SIGN enables the highly efficient migration of dendritic cells from blood into the tissues. It can interact with ICAM-2, which has a similar sequence as ICAM-3, and is abundantly expressed on vascular and lymphoid endothelium. Thus, DC-SIGN mediates dendritic cells rolling and transendothelial migration, and its interaction with ICAM-2 is essential to specific migratory functions of dendritic cells.
研发参考 (5)
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Appelmelk BJ, van Die I, van Vliet SJ, et al. Carbohydrate profiling identifies new pathogens that interact with dendritic cell-specific ICAM-3-grabbing nonintegrin on dendritic cells. J Immunol. 2003; 170(4):1635-1639. (Biology). 查看参考
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Gruber A, Chalmers AS, Popov S, Ruprecht RM. Functional aspects of binding of monoclonal antibody DCN46 to DC-SIGN on dendritic cells.. Immunol Lett. 2002; 84(2):103-8. (Clone-specific). 查看参考
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Sallusto F, Cella M, Danieli C, Lanzavecchia A. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products. J Exp Med. 1995; 182(2):389-400. (Immunogen). 查看参考
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Steinman RM, Granelli-Piperno A, Pope M, et al. The interaction of immunodeficiency viruses with dendritic cells. Curr Top Microbiol Immunol. 2003; 276:1-30. (Biology). 查看参考
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Steinman RM. DC-SIGN: a guide to some mysteries to dendritic cells. Cell. 2000; 100(5):491-494. (Biology). 查看参考
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