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Enhance your research with PerCP-Cy5.5 alternative with reduced spillover and monocyte background! Try BD Horizon RealBlue™ 705 SKU# 757509 today! More Info on RB705 #
550787test.png

Multicolor flow cytometric analysis of CD14 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PerCP-Cy5.5 Mouse IgG2a, κ Isotype Control (Cat. No. 550927; Left Plot) or PerCP-Cy5.5 Mouse Anti-Human CD14 antibody (Cat. No. 550787; Right Plot) at 1.0 µg/test. Erythrocytes were lysed with BD Phamr Lyse™ Lysing Buffer (Cat. No. 555899). Bivariate pseudocolor density plots showing CD14 expression (or Ig Isotype staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

550787Image1.png

Multicolor flow cytometric analysis of CD14 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PerCP-Cy5.5 Mouse IgG2a, κ Isotype Control (Cat. No. 550927; Left Plot) or PerCP-Cy5.5 Mouse Anti-Human CD14 antibody (Cat. No. 550787; Right Plot) at 1.0 µg/test. Erythrocytes were lysed with BD Phamr Lyse™ Lysing Buffer (Cat. No. 555899). Bivariate pseudocolor density plots showing CD14 expression (or Ig Isotype staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

550787test.png 3054TA_550787_image1.png 550787Image1.png Amber-Cap-Blue-Label.jpg

Multicolor flow cytometric analysis of CD14 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PerCP-Cy5.5 Mouse IgG2a, κ Isotype Control (Cat. No. 550927; Left Plot) or PerCP-Cy5.5 Mouse Anti-Human CD14 antibody (Cat. No. 550787; Right Plot) at 1.0 µg/test. Erythrocytes were lysed with BD Phamr Lyse™ Lysing Buffer (Cat. No. 555899). Bivariate pseudocolor density plots showing CD14 expression (or Ig Isotype staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Multicolor flow cytometric analysis of CD14 expression on human peripheral blood leucocyte populations. Human whole blood was stained with either PerCP-Cy5.5 Mouse IgG2a, κ Isotype Control (Cat. No. 550927; Left Plot) or PerCP-Cy5.5 Mouse Anti-Human CD14 antibody (Cat. No. 550787; Right Plot) at 1.0 µg/test. Erythrocytes were lysed with BD Phamr Lyse™ Lysing Buffer (Cat. No. 555899). Bivariate pseudocolor density plots showing CD14 expression (or Ig Isotype staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

商品详情
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BD Pharmingen™
LPS receptor; LPS-R; Myeloid cell-specific leucine-rich glycoprotein
Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog (Tested in Development)
Mouse IgG2a, κ
Human CD14 Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
II M34; III M329
AB_393884
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
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推荐的实验流程

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
  6. PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  10. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
  11. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
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550787 Rev. 2
抗体详情
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M5E2

The M5E2 monoclonal antibody specifically binds to CD14, a 53–55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes. Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells. CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

550787 Rev. 2
格式详情
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PerCP-Cy5.5
482 nm
676 nm
550787 Rev.2
报价单和参考
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View product citations for antibody "550787" on CiteAb

研发参考 (5)

  1. Bernstein ID, Self S. Joint report of the Myeloid Section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1986:1-25.
  2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  3. McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050.
  4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  5. Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990; 249(4975):1431-1433. (Biology). 查看参考
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550787 Rev. 2

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.