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      PE Rat anti-Pax-5
      PE Rat anti-Pax-5
      Multicolor flow cytometric analysis of Pax5 expression in human and mouse lymphocytes. Human peripheral blood mononuclear cells (PBMC) and BALB/c mouse splenocytes were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574) and then stained with either PE Rat Anti-Mouse Pax5 antibody (Cat. No. 562688) or PE rat IgG2a, κ Isotype Control (Cat. No. 554689). The PBMC were counterstained with BD Horizon™ V450 Mouse Anti-Human CD3 (Cat. No. 560366) and APC Mouse Anti-Human CD21 (Cat. No. 559867) antibodies. Mouse splenocytes were counterstained with PerCP-Cy™5.5 Rat Anti-Mouse CD45R/B220 (Cat. No. 552771) and FITC Hamster Anti-Mouse CD3e (Cat. No. 553062) antibodies. Upper Panel: Human Peripheral Blood Lymphocytes (PBL): Contour plots from left to right showing the expression of CD21 versus Rat IgG2a κ Isotype Control, CD21 versus Pax5 and CD3 versus Pax5, respectively. Lower Panel: Mouse Splenic Lymphocytes: Contour plots from left to right showing the expression of CD45R/B220 versus Rat IgG2a κ Isotype Control, CD45R/B220 versus PE Pax5 and CD3e versus Pax5, respectively. The flow cytometric data were derived from gated events with the forward and side light-scatter characteristics of intact human (top plots) and mouse (bottom plots) lymphocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
      PE Rat anti-Pax-5
      Multicolor flow cytometric analysis of Pax5 expression in human and mouse lymphocytes. Human peripheral blood mononuclear cells (PBMC) and BALB/c mouse splenocytes were fixed and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574) and then stained with either PE Rat Anti-Mouse Pax5 antibody (Cat. No. 562688) or PE rat IgG2a, κ Isotype Control (Cat. No. 554689). The PBMC were counterstained with BD Horizon™ V450 Mouse Anti-Human CD3 (Cat. No. 560366) and APC Mouse Anti-Human CD21 (Cat. No. 559867) antibodies. Mouse splenocytes were counterstained with PerCP-Cy™5.5 Rat Anti-Mouse CD45R/B220 (Cat. No. 552771) and FITC Hamster Anti-Mouse CD3e (Cat. No. 553062) antibodies. Upper Panel: Human Peripheral Blood Lymphocytes (PBL): Contour plots from left to right showing the expression of CD21 versus Rat IgG2a κ Isotype Control, CD21 versus Pax5 and CD3 versus Pax5, respectively. Lower Panel: Mouse Splenic Lymphocytes: Contour plots from left to right showing the expression of CD45R/B220 versus Rat IgG2a κ Isotype Control, CD45R/B220 versus PE Pax5 and CD3e versus Pax5, respectively. The flow cytometric data were derived from gated events with the forward and side light-scatter characteristics of intact human (top plots) and mouse (bottom plots) lymphocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometer System.
      商品详情
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      BD Pharmingen™
      Pax5, PAX5, KLP, BSAP, EBB-1; B-cell-specific transcription factor
      Mouse (QC Testing), Human (Tested in Development)
      Rat IgG2a, κ
      Recombinant Mouse Pax-5 protein containing aa 154-284
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      AB_2737722
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      准备和存储

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      562688 Rev. 1
      抗体详情
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      1H9

      The 1H9 monoclonal antibody clone specifically binds to human and mouse Paired box protein Pax-5. Pax-5 is a ~50 kDa protein that is also known as B-cell-specific transcription factor and B cell specific activator protein (BSAP). Pax-5 is a member of the paired box (Pax) family of transcription factors. It is expressed in pro-B, pre-B and mature B cells. Through the Groucho family of co-repressors, Pax-5 likely functions as a transcriptional repressor of non-B-lymphoid genes during the B cell commitment process. In the early stages of B cell development, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20 and maintains B cell identity. Pax-5 suppression is involved in the upregulation of Blimp-1 leading to the development of Pax-5-negative plasma cells. Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. Altered forms and expression patterns of Pax-5 have been associated with some lymphoid and nonlymphoid cancers.

      562688 Rev. 1
      格式详情
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      562688 Rev.1
      报价单和参考
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      研发参考 (5)

      1. Adams B, Dorfler P, Aguzzi A, et al. Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis. Genes Dev. 1992; 6(9):1589-1607. (Biology). 查看参考
      2. Kallies A, Hasbold J, Fairfax K, et al. Initiation of plasma-cell differentiation is independent of the transcription factor Blimp-1. Immunity. 2007; 26(5):555-566. (Immunogen: Western blot). 查看参考
      3. Klein U, Tu Y, Stolovitzky GA, et al. Transcriptional analysis of the B cell germinal center reaction. Proc Natl Acad Sci U S A. 2003; 100(5):2639-2644. (Biology). 查看参考
      4. O'Brien P, Morin P, Jr., Ouellette RJ, Robichaud GA. The Pax-5 gene: a pluripotent regulator of B-cell differentiation and cancer disease. Cancer Res. 2011; 71(24):7345-7350. (Biology). 查看参考
      5. Zwollo P, Arrieta H, Ede K, Molinder K, Desiderio S, Pollock R. The Pax-5 gene is alternatively spliced during B-cell development. J Biol Chem. 1997; 272(15):10160-10168. (Biology). 查看参考
      查看所有文件 (5) 查看更少内容
      562688 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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